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Fermentation 2017, 3(2), 18; doi:10.3390/fermentation3020018

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

1
Department of Animal Sciences, The Ohio State University, and Ohio State Agricultural Research and Development Center (OARDC), 305 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH 44691, USA
2
Renewable Energy Program, Agricultural Technical Institute, The Ohio State University, 1328 Dover Road, Wooster, OH 44691, USA
3
Crop Production Division, ICAR—Vivekananda Parvatiya Krishi Anusandhan Sansthan, Department of Agricultural Research and Education (DARE), Ministry of Agriculture and Farmers Welfare, Government of India), Almora 263601, Uttarakhand, India
*
Authors to whom correspondence should be addressed.
Academic Editor: Badal C. Saha
Received: 9 March 2017 / Revised: 21 April 2017 / Accepted: 2 May 2017 / Published: 7 May 2017
(This article belongs to the Special Issue Biofuels and Biochemicals Production)
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Abstract

While chiral 2,3-Butanediol (2,3-BD) is currently receiving remarkable attention because of its numerous industrial applications in the synthetic rubber, bioplastics, cosmetics, and flavor industries, 2,3-BD-mediated feedback inhibition of Paenibacillus polymyxa DSM 365 limits the accumulation of higher concentrations of 2,3-BD in the bioreactor during fermentation. The Box-Behnken design, Plackett-Burman design (PBD), and response surface methodology were employed to evaluate the impacts of seven factors including tryptone, yeast extract, ammonium acetate, ammonium sulfate, glycerol concentrations, temperature, and inoculum size on 2,3-butanediol (2,3-BD) production by Paenibacillus polymyxa DSM 365. Results showed that three factors; tryptone, temperature, and inoculum size significantly influence 2,3-BD production (p < 0.05) by P. polymyxa. The optimal levels of tryptone, inoculum size, and temperature as determined by the Box-Behnken design and response surface methodology were 3.5 g/L, 9.5%, and 35 °C, respectively. The optimized process was validated in batch and fed-batch fermentations in a 5-L Bioflo 3000 Bioreactor, and 51.10 and 68.54 g/L 2,3-BD were obtained, respectively. Interestingly, the production of exopolysaccharides (EPS), an undesirable co-product, was reduced by 19% when compared to the control. These results underscore an interplay between medium components and fermentation conditions, leading to increased 2,3-BD production and decreased EPS production by P. polymyxa. Collectively, our findings demonstrate both increased 2,3-BD titer, a fundamental prerequisite to the potential commercialization of fermentative 2,3-BD production using renewable feedstocks, and reduced flux of carbons towards undesirable EPS production. View Full-Text
Keywords: Paenibacillus polymyxa; butanediol; acetoin; glycerol; optimization Paenibacillus polymyxa; butanediol; acetoin; glycerol; optimization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Okonkwo, C.C.; Ujor, V.C.; Mishra, P.K.; Ezeji, T.C. Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365. Fermentation 2017, 3, 18.

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