Next Article in Journal
Co-Cultivation of Penicillium sp. AKB-24 and Aspergillus nidulans AKB-25 as a Cost-Effective Method to Produce Cellulases for the Hydrolysis of Pearl Millet Stover
Next Article in Special Issue
Application of Non-Saccharomyces Yeasts to Wine-Making Process
Previous Article in Journal
Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation
Previous Article in Special Issue
The Interaction of Two Saccharomyces cerevisiae Strains Affects Fermentation-Derived Compounds in Wine
Article Menu

Export Article

Open AccessArticle
Fermentation 2016, 2(2), 11; doi:10.3390/fermentation2020011

A Bacterial Laccase for Enhancing Saccharification and Ethanol Fermentation of Steam-Pretreated Biomass

1
IMDEA Energía, Biotechnological Processes for Energy Production Unit, Móstoles, Madrid 28935, Spain
2
CIEMAT, Renewable Energy Division, Biofuels Unit, Avda. Complutense 40, Madrid 28040, Spain
3
INIA-CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra de La Coruña Km 7.5, Madrid 28040, Spain
4
MetGen Oy, Rakentajantie 26, Kaarina 20780, Finland
*
Author to whom correspondence should be addressed.
Academic Editor: Ronnie G. Willaert
Received: 8 April 2016 / Revised: 22 April 2016 / Accepted: 26 April 2016 / Published: 4 May 2016
(This article belongs to the Special Issue Yeast Biotechnology)
View Full-Text   |   Download PDF [771 KB, uploaded 4 May 2016]   |  

Abstract

Different biological approaches, highlighting the use of laccases, have been developed as environmentally friendly alternatives for improving the saccharification and fermentation stages of steam-pretreated lignocellulosic biomass. This work evaluates the use of a novel bacterial laccase (MetZyme) for enhancing the hydrolysability and fermentability of steam-exploded wheat straw. When the water insoluble solids (WIS) fraction was treated with laccase or alkali alone, a modest increase of about 5% in the sugar recovery yield (glucose and xylose) was observed in both treatments. Interestingly, the combination of alkali extraction and laccase treatment boosted enzymatic hydrolysis, increasing the glucose and xylose concentration in the hydrolysate by 21% and 30%, respectively. With regards to the fermentation stage, the whole pretreated slurry was subjected to laccase treatment, lowering the phenol content by up to 21%. This reduction allowed us to improve the fermentation performance of the thermotolerant yeast Kluyveromyces marxianus CECT 10875 during a simultaneous saccharification and fermentation (SSF) process. Hence, a shorter adaptation period and an increase in the cell viability—measured in terms of colony forming units (CFU/mL)—could be observed in laccase-treated slurries. These differences were even more evident when a presaccharification step was performed prior to SSF. Novel biocatalysts such as the bacterial laccase presented in this work could play a key role in the implementation of a cost-effective technology in future biorefineries. View Full-Text
Keywords: alkaline extraction; bacterial Metzyme laccase; lignocellulosic ethanol; simultaneous saccharification and fermentation; thermotolerant yeast alkaline extraction; bacterial Metzyme laccase; lignocellulosic ethanol; simultaneous saccharification and fermentation; thermotolerant yeast
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Moreno, A.D.; Ibarra, D.; Mialon, A.; Ballesteros, M. A Bacterial Laccase for Enhancing Saccharification and Ethanol Fermentation of Steam-Pretreated Biomass. Fermentation 2016, 2, 11.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Fermentation EISSN 2311-5637 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top