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Fermentation 2016, 2(2), 10; doi:10.3390/fermentation2020010

Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

Microbiology Research Laboratory, Department of Basic Sciences, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan-173230, HP, India
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Academic Editor: Marc Beauregard
Received: 19 January 2016 / Revised: 19 March 2016 / Accepted: 19 March 2016 / Published: 4 May 2016
(This article belongs to the Special Issue Industrial Biotechnology: An Emerging Area)
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Abstract

A novel strain Rhizopus sp. C4 was isolated from compost for the production of pectinase. Cultivation of Rhizopus sp. C4 on orange peel substrate under various solid-state fermentation (SSF) conditions was evaluated for pectinase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. Response surface methodology (RSM) was employed to optimize various environmental parameters for pectinase production. Various parameters, namely temperature, moisture and incubation days, were studied statistically for a total of 20 runs using central composite design. The highest yield of the enzyme, i.e., 11.63 IU/mL, was obtained from 1:3.5 moisture ratios in 7 days at 30°C. The study demonstrated that optimization through RSM could improve the enzymatic characteristics and yield of the enzyme. View Full-Text
Keywords: pectinases; enzyme; optimization; pectinase; solid state fermentation pectinases; enzyme; optimization; pectinase; solid state fermentation
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Handa, S.; Sharma, N.; Pathania, S. Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation. Fermentation 2016, 2, 10.

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