Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Substrates and Chemicals
2.2. Source of Fungal Inoculum
2.3. Solid State Fermentation
2.4. Polygalacturonase (PG) Activity
2.5. Experimental Design for Optimization
2.6. Model Validation
3. Results and Discussion
3.1. Cultural Characteristics of Fungal Isolate
3.2. Quantitative Estimation of Pectinase
3.3. Optimization of Culture Conditions Using Response Surface Methodology
3.4. Validation of the Model
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Factors | Unit | Symbols | Actual Level Coded Factor | |
---|---|---|---|---|
−1 | 1 | |||
Temperature | °C | A | 20 | 40 |
Moisture | % | B | 1:1 | 1:5 |
Incubation Time | Days | C | 4 | 12 |
Run | A:Temperature | B:moisture | C:Incubation Time | Enzyme Units (IU/mL) | |
---|---|---|---|---|---|
Experimental Values | Predicted Value | ||||
1 | 40.00 | 1:5.00 | 10.00 | 2.90 | 2.62 |
2 | 30.00 | 1: 3.50 | 7.00 | 11.63 | 11.61 |
3 | 30.00 | 1:0.98 | 7.00 | 3.40 | 3.43 |
4 | 30.00 | 1:3.50 | 12.05 | 1.93 | 2.60 |
5 | 20.00 | 1:2.00 | 10.00 | 2.11 | 2.00 |
6 | 40.00 | 1:2.00 | 4.00 | 3.50 | 3.75 |
7 | 30.00 | 1:3.50 | 7.00 | 11.63 | 11.61 |
8 | 13.18 | 1:3.50 | 7.00 | 2.33 | 2.43 |
9 | 30.00 | 1:3.50 | 7.00 | 11.63 | 11.61 |
10 | 30.00 | 1:6.02 | 7.00 | 5.07 | 5.60 |
11 | 20.00 | 1:5.00 | 10.00 | 5.81 | 5.16 |
12 | 40.00 | 1:2.00 | 10.00 | 0.90 | 0.47 |
13 | 40.00 | 1:5.00 | 4.00 | 3.49 | 3.18 |
14 | 20.00 | 1:2.00 | 4.00 | 2.11 | 2.02 |
15 | 30.00 | 1:3.50 | 7.00 | 11.63 | 11.61 |
16 | 46.82 | 1:3.50 | 7.00 | 1.70 | 1.96 |
17 | 30.00 | 1:3.50 | 7.00 | 11.63 | 11.61 |
18 | 20.00 | 1:5.00 | 4.00 | 2.40 | 2.43 |
19 | 30.00 | 1:3.50 | 1.95 | 3.17 | 3.06 |
Source | Sum of Squares | DF | Mean Value | F Square | Prob> F |
---|---|---|---|---|---|
Model | 300.42 | 9 | 33.38 | 170.45 | <0.0001 * |
A | 0.53 | 1 | 0.53 | 2.72 | 0.1332 |
B | 5.66 | 1 | 5.66 | 28.88 | 0.0004 * |
C | 0.25 | 1 | 0.25 | 1.30 | 0.2835 |
A2 | 148.07 | 1 | 148.07 | 756.08 | <0.0001 * |
B2 | 85.90 | 1 | 85.90 | 438.65 | <0.0001 * |
C2 | 131.55 | 1 | 131.55 | 671.73 | <0.0001 * |
AB | 0.50 | 1 | 0.50 | 2.55 | 0.1445 |
AC | 5.44 | 1 | 5.44 | 27.80 | 0.0005 * |
BC | 3.67 | 1 | 3.67 | 18.75 | 0.0019 * |
Residual | 1.76 | 9 | 0.20 | ||
Lack of Fit | 1.76 | 5 | 0.35 | ||
Pure Error | 0.000 | 4 | 0.000 | ||
Cor Total | 302.18 | 18 |
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Handa, S.; Sharma, N.; Pathania, S. Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation. Fermentation 2016, 2, 10. https://doi.org/10.3390/fermentation2020010
Handa S, Sharma N, Pathania S. Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation. Fermentation. 2016; 2(2):10. https://doi.org/10.3390/fermentation2020010
Chicago/Turabian StyleHanda, Shweta, Nivedita Sharma, and Shruti Pathania. 2016. "Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation" Fermentation 2, no. 2: 10. https://doi.org/10.3390/fermentation2020010