Next Article in Journal
Current perspectives in Set7 mediated stem cell differentiation
Previous Article in Journal
TWEAK Negatively Regulates Human Dicer
Article Menu

Export Article

Open AccessCommunication
Non-Coding RNA 2016, 2(4), 13; doi:10.3390/ncrna2040013

High Percentage of Isomeric Human MicroRNA and Their Analytical Challenges

Department of Chemistry and Biochemistry, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27412, USA
*
Author to whom correspondence should be addressed.
Academic Editor: George A. Calin
Received: 16 September 2016 / Revised: 25 October 2016 / Accepted: 23 November 2016 / Published: 2 December 2016
View Full-Text   |   Download PDF [13600 KB, uploaded 2 December 2016]   |  

Abstract

MicroRNA (miR) are short non-coding RNAs known to post-transcriptionally regulate gene expression, and have been reported as biomarkers for various diseases. miR have also been served as potential drug targets. The identity, functions and detection of a specific miR are determined by its RNA sequence, whose composition is made up of only 4 canonical ribonucleotides. Hence, among over two thousand human miR, their nucleotide compositions are expected to be similar but the extent of similarity has not been reported. In this study, the sequences of mature human miR were downloaded from miRBase, and collated using different tools to determine and compare their nucleotide compositions and sequences. 55% of all human miR were found to be structural isomers. The structural isomers of miR (SimiR) are defined as having the same size and identical nucleotide composition. A number of SimiR were also found to have high sequence similarities. To investigate the extent of SimiR in biological samples, three disease models were chosen, and disease-associated miR were identified from miR2Disease. Among the disease models, as high as 73% of miR were found to be SimiR. This report provides the missing information about human miR and highlights the challenges on the detection of SimiR. View Full-Text
Keywords: microRNA; nucleotide composition; isomers microRNA; nucleotide composition; isomers
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mwangi, J.N.; Chiu, N.H.L. High Percentage of Isomeric Human MicroRNA and Their Analytical Challenges. Non-Coding RNA 2016, 2, 13.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Non-Coding RNA EISSN 2311-553X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top