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Non-Coding RNA 2015, 1(3), 170-191; doi:10.3390/ncrna1030170

Epstein-Barr Virus EBER Transcripts Affect miRNA-Mediated Regulation of Specific Targets and Are Processed to Small RNA Species

1
Institute of Virology, Saarland University Medical School, Kirrbergerstrasse, Haus 47, Homburg/Saar D-66421, Germany
2
Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, Universitätsstrasse 31, Regensburg D-93053, Germany
3
College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
4
Max Planck Genome Centre Cologne, Carl-von-Linné-Weg 10, Cologne D-50829, Germany
*
Authors to whom correspondence should be addressed.
Academic Editor: George A. Calin
Received: 11 August 2015 / Revised: 22 September 2015 / Accepted: 22 September 2015 / Published: 30 September 2015
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Abstract

The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5′-derived RNAs of approximately 23, 52 and 70 nt (EBER123, EBER152, and EBER170) and of EBER2 to 3′ fragments. The biogenesis of these species is independent of Dicer, and EBER123 does not act like a miRNA OPEN ACCESS Non-Coding RNA 2015, 1 171 to target its complementary sequence. EBER1, EBER2 and EBER123 were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs. View Full-Text
Keywords: Epstein-Barr virus; EBV; EBER; microRNA; DICER; Lupus antigen; La; ebv-miR-BART16; hsa-miR-142; IL1A; RAC1; TOMM22; ADCY9 Epstein-Barr virus; EBV; EBER; microRNA; DICER; Lupus antigen; La; ebv-miR-BART16; hsa-miR-142; IL1A; RAC1; TOMM22; ADCY9
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MDPI and ACS Style

Alles, J.; Hasler, D.; Kazmi, S.M.A.; Tesson, M.; Hamilton, A.; Schlegel, L.; Marx, S.; Eichner, N.; Reinhardt, R.; Meister, G.; Wilson, J.B.; Grässer, F.A. Epstein-Barr Virus EBER Transcripts Affect miRNA-Mediated Regulation of Specific Targets and Are Processed to Small RNA Species. Non-Coding RNA 2015, 1, 170-191.

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