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Non-Coding RNA 2015, 1(2), 127-138; doi:10.3390/ncrna1020127

“Pocket-sized RNA-Seq”: A Method to Capture New Mature microRNA Produced from a Genomic Region of Interest

1
University Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France
2
Epigénétique et Destin Cellulaire, CNRS UMR 7216, 75013 Paris, France
*
Authors to whom correspondence should be addressed.
Academic Editor: George A. Calin
Received: 11 May 2015 / Revised: 3 June 2015 / Accepted: 23 June 2015 / Published: 3 July 2015
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Abstract

Currently, the discovery of new small ncRNAs requires high throughput methods even in the case of focused research on the regulation of specific genes or set of genes. We propose herein a simple, rapid, efficient, and cost effective method to clone and sequence single, yet unknown, small ncRNA. This technique that we called “Pocket-sized RNA-Seq” or psRNA-seq is based on in vitro transcription, RNA pull down and adapted RACE-PCR methods that allow its implementation using either available commercial kits or in-house reagents. View Full-Text
Keywords: RNA pull down; RACE-PCR; hsa-miR-21; hsa-miR-1237; mirtron; miRNA; mature miRNA sequence RNA pull down; RACE-PCR; hsa-miR-21; hsa-miR-1237; mirtron; miRNA; mature miRNA sequence
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hubé, F.; Francastel, C. “Pocket-sized RNA-Seq”: A Method to Capture New Mature microRNA Produced from a Genomic Region of Interest. Non-Coding RNA 2015, 1, 127-138.

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