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Non-Coding RNA 2015, 1(2), 94-126; doi:10.3390/ncrna1020094

Expression of Subtelomeric lncRNAs Links Telomeres Dynamics to RNA Decay in S. cerevisiae

1
RNA, epigenetics and genome fluidity, Institut Curie, PSL Research University, CNRS UMR3244, Université Pierre et Marie Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France
2
Nuclear Dynamics, Institut Curie, PSL Research University, CNRS UMR3664, Université Pierre et Marie Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France
Current address: Institut de Biologie Intégrative de la Cellule, CNRS UMR9198, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France
Current address: Dynamic nuclear organization, Laboratoire de Biologie Moléculaire Eucaryote, CNRS UMR5099, 118 Route de Narbonne, 31062 Toulouse Cedex 9, France.
*
Authors to whom correspondence should be addressed.
Academic Editor: George A. Calin
Received: 20 April 2015 / Revised: 17 June 2015 / Accepted: 17 June 2015 / Published: 3 July 2015
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Abstract

Long non-coding RNAs (lncRNAs) have been shown to regulate gene expression, chromatin domains and chromosome stability in eukaryotic cells. Recent observations have reported the existence of telomeric repeats containing long ncRNAs – TERRA in mammalian and yeast cells. However, their functions remain poorly characterized. Here, we report the existence in S. cerevisiae of several lncRNAs within Y′ subtelomeric regions. We have called them subTERRA. These belong to Cryptic Unstable Transcripts (CUTs) and Xrn1p-sensitive Unstable Transcripts (XUTs) family. subTERRA transcription, carried out mainly by RNAPII, is initiated within the subtelomeric Y’ element and occurs in both directions, towards telomeres as well as centromeres. We show that subTERRA are distinct from TERRA and are mainly degraded by the general cytoplasmic and nuclear 5′- and 3′- RNA decay pathways in a transcription-dependent manner. subTERRA accumulates preferentially during the G1/S transition and in C-terminal rap1 mutant but independently of Rap1p function in silencing. The accumulation of subTERRA in RNA decay mutants coincides with telomere misregulation: shortening of telomeres, loss of telomeric clustering in mitotic cells and changes in silencing of subtelomeric regions. Our data suggest that subtelomeric RNAs expression links telomere maintenance to RNA degradation pathways. View Full-Text
Keywords: subtelomeric region; non-coding RNA; telomere maintenance; S. cerevisiae; Rap1p subtelomeric region; non-coding RNA; telomere maintenance; S. cerevisiae; Rap1p
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Kwapisz, M.; Ruault, M.; van Dijk, E.; Gourvennec, S.; Descrimes, M.; Taddei, A.; Morillon, A. Expression of Subtelomeric lncRNAs Links Telomeres Dynamics to RNA Decay in S. cerevisiae. Non-Coding RNA 2015, 1, 94-126.

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