Multifunctional Drugs-Loaded Carbomol Hydrogel Promotes Diabetic Wound Healing via Antimicrobial and Immunoregulation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of Hydrogels
2.2. Cell Biocompatibility and Antibacterial/Antibiofilm Activities of Hydrogels
2.3. QVR-CBMG Hydrogel Affected the Polarization of Macrophages
2.4. QVR-CBMG Hydrogel Activated the Sirtuin 3(SIRT3)/Superoxide Dismutase (SOD2) Pathway
2.5. QVR-CBMG Hydrogel Accelerates Diabetic Infection Rat Wound Healing
2.6. QVR-CBMG Hydrogel Promoted Collagen Repair and Angiogenesis In Vivo
2.7. QVR-CBMG Hydrogel Facilitates M2 Polarization of Macrophages In Vivo
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Preparation of Hydrogels
4.3. Scanning Electron Microscope (SEM) Analysis
4.4. Rheological Experiment
4.5. Cell Biocompatibilitye
4.6. Macrophages Extraction and Culture
4.7. Immunofluorescence (IF) Staining
4.8. Western Blot (WB) Assay
4.9. Quantitative Polymerase Chain Reaction (qPCR)
4.10. Antimicrobial Activity Tests In Vitro
4.11. Rats Model and Treatment
4.12. Histological and Immunostaining Analyses
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, H.; Jin, J.; Zhang, C.; Gong, F.; Hu, B.; Wu, X.; Guan, M.; Xia, D. Multifunctional Drugs-Loaded Carbomol Hydrogel Promotes Diabetic Wound Healing via Antimicrobial and Immunoregulation. Gels 2023, 9, 761. https://doi.org/10.3390/gels9090761
Wang H, Jin J, Zhang C, Gong F, Hu B, Wu X, Guan M, Xia D. Multifunctional Drugs-Loaded Carbomol Hydrogel Promotes Diabetic Wound Healing via Antimicrobial and Immunoregulation. Gels. 2023; 9(9):761. https://doi.org/10.3390/gels9090761
Chicago/Turabian StyleWang, Hehui, Jiale Jin, Chi Zhang, Fangyi Gong, Baiwen Hu, Xiaochuan Wu, Ming Guan, and Dongdong Xia. 2023. "Multifunctional Drugs-Loaded Carbomol Hydrogel Promotes Diabetic Wound Healing via Antimicrobial and Immunoregulation" Gels 9, no. 9: 761. https://doi.org/10.3390/gels9090761