Biocompatible Anisole-Nonlinear PEG Core–Shell Nanogels for High Loading Capacity, Excellent Stability, and Controlled Release of Curcumin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Size and Morphology of the PVAS@PEG Core–Shell Nanogels
2.2. Thermo-Responsive Volume Phase Transitions of the PVAS@PEG Nanogels
2.3. Curcumin Loading Capacity of the PVAS@PEG Nanogels
2.4. Curcumin Stability in the PVAS@PEG Nanogels
2.5. Thermo-Responsive Curcumin Release from the PVAS@PEG Nanogels
2.6. Cellular Internalization of the PVAS@PEG Nanogels
2.7. In Vitro Cytocompatibility of the PVAS@PEG Nanogels
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Synthesis of PVAS Core Nanogels
4.3. Synthesis of PVAS@PEG Core–Shell Nanogels
4.4. Curcumin Loading and Release
4.5. Internalization of Nanogels into Mouse Melanoma Cells B16F10
4.6. In Vitro Cytotoxicity
4.7. Characterization
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Salehi, B.; Stojanovic-Radic, Z.; Matejic, J.; Sharifi-Rad, M.; Kumar, N.V.A.; Martins, N.; Sharifi-Rad, J. The therapeutic potential of curcumin: A review of clinical trials. Eur. J. Med. Chem. 2019, 163, 527–545. [Google Scholar] [CrossRef] [PubMed]
- Kunnumakkara, A.B.; Hegde, M.; Parama, D.; Girisa, S.; Kumar, A.; Daimary, U.D.; Garodia, P.; Yenisetti, S.C.; Oommen, O.V.; Aggarwal, B.B. Role of Turmeric and Curcumin in Prevention and Treatment of Chronic Diseases: Lessons Learned from Clinical Trials. ACS Pharmacol. Transl. Sci. 2023, 6, 447–518. [Google Scholar] [CrossRef]
- Karthikeyan, A.; Senthil, N.; Min, T. Nanocurcumin: A Promising Candidate for Therapeutic Applications. Front. Pharmacol. 2020, 11, 487. [Google Scholar] [CrossRef]
- Salehi, B.; Quispe, C.; Chamkhi, I.; Omari, N.E.; Balahbib, A.; Sharifi-Rad, J.; Bouyahya, A.; Akram, M.; Iqbal, M.; Docea, A.O.; et al. Pharmacological Properties of Chalcones: A Review of Preclinical Including Molecular Mechanisms and Clinical Evidence. Front. Pharmacol. 2021, 11, 592654. [Google Scholar] [CrossRef] [PubMed]
- Zhang, D.; Fu, M.; Gao, S.; Liu, J. Curcumin and Diabetes: A Systematic Review. Evid. Based Complement. Alternat. Med. 2013, 2013, 636053. [Google Scholar] [CrossRef]
- Marton, L.T.; Pescinini-e-Salzedas, L.M.; Camargo, M.E.C.; Barbalho, S.M.; Haber, J.F.D.S.; Sinatora, R.V.; Detregiachi, C.R.P.; Girio, R.J.S.; Buchaim, D.V.; Bueno, P.C.D.S. The Effects of Curcumin on Diabetes Mellitus: A Systematic Review. Front. Endocrinol. 2021, 12, 669448. [Google Scholar] [CrossRef] [PubMed]
- Quispe, C.; Herrera-Bravo, J.; Javed, Z.; Khan, K.; Raza, S.; Gulsunoglu-Konuskan, Z.; Daştan, S.D.; Sytar, O.; Martorell, M.; Sharifi-Rad, J.; et al. Therapeutic Applications of Curcumin in Diabetes: A Review and Perspective. BioMed. Res. Int. 2022, 2022, 1375892. [Google Scholar] [CrossRef]
- Bozkurt, O.; Kocaadam-Bozkurt, B.; Yildiran, H. Effects of curcumin, a bioactive component of turmeric, on type 2 diabetes mellitus and its complications: An updated review. Food Funct. 2022, 13, 11999–12010. [Google Scholar] [CrossRef] [PubMed]
- Lu, W.; Shahidi, F.K.; Khorsandi, K.; Hosseinzadeh, R.; Gul, A.; Balick, V. An update on molecular mechanisms of curcumin effect on diabetes. J. Food Biochem. 2022, 46, e14358. [Google Scholar] [CrossRef]
- Hodaei, H.; Adibian, M.; Nikpayam, O.; Hedayati, M.; Sohrab, G. The effect of curcumin supplementation on anthropometric indices, insulin resistance and oxidative stress in patients with type 2 diabetes: A randomized, double-blind clinical trial. Diabetol. Metab. Syndr. 2019, 11, 41. [Google Scholar] [CrossRef]
- Pivari, F.; Mingione, A.; Brasacchio, C.; Soldati, L. Curcumin and Type 2 Diabetes Mellitus: Prevention and Treatment. Nutrients 2019, 11, 1837. [Google Scholar] [CrossRef] [PubMed]
- Roxo, D.F.; Arcaro, C.A.; Gutierres, V.O.; Costa, M.C.; Oliveira, J.O.; Lima, T.F.O.; Assis, R.P.; Brunetti, I.L.; Baviera, A.M. Curcumin combined with metformin decreases glycemia and dyslipidemia, and increases paraoxonase activity in diabetic rats. Diabetol. Metab. Syndr. 2019, 11, 33. [Google Scholar] [CrossRef] [PubMed]
- Rivera-Mancía, S.; Trujillo, J.; Chaverri, J.P. Utility of curcumin for the treatment of diabetes mellitus: Evidence from preclinical and clinical studies. J. Nutr. Intermed. Metab. 2018, 14, 29–41. [Google Scholar] [CrossRef]
- Wang, L.; Xu, J.; Yu, T.; Wang, H.; Cai, X.; Sun, H. Efficacy and safety of curcumin in diabetic retinopathy: A protocol for systematic review and meta-analysis. PLoS ONE 2023, 18, e0282866. [Google Scholar] [CrossRef] [PubMed]
- Gupta, S.C.; Kismali, G.; Aggarwal, B.B. Curcumin, a component of turmeric: From farm to pharmacy. Biofactors 2013, 39, 2–13. [Google Scholar] [CrossRef]
- Anand, P.; Kunnumakkara, A.B.; Newman, R.A.; Aggarwal, B.B. Bioavailability of curcumin: Problems and promises. Mol. Pharm. 2007, 4, 807–818. [Google Scholar] [CrossRef]
- Naksuriya, O.; Okonogi, S.; Schiffelers, R.M.; Hennink, W.E. Curcumin nanoformulations: A review of pharmaceutical properties and preclinical studies and clinical data related to cancer treatment. Biomaterials 2014, 35, 3365–3383. [Google Scholar] [CrossRef]
- Sharma, R.A.; Euden, S.A.; Platton, S.L.; Cooke, D.N.; Shafayat, A.; Hewitt, H.R.; Marczylo, T.H.; Morgan, B.; Hemingway, D.; Plummer, S.M.; et al. Phase I clinical trial of oral curcumin: Biomarkers of systemic activity and compliance. Clin. Cancer Res. 2004, 10, 6847–6854. [Google Scholar] [CrossRef]
- Storka, A.; Vcelar, B.; Klickovic, U.; Gouya, G.; Weisshaar, S.; Aschauer, S.; Bolger, G.; Helson, L.; Wolzt, M. Safety, tolerability and pharmacokinetics of liposomal curcumin in healthy humans. Int. J. Clin. Pharmacol. Ther. 2015, 53, 54–65. [Google Scholar] [CrossRef]
- Pourmadadi, M.; Abbasi, P.; Eshaghi, M.M.; Bakhshi, A.; Manicum, A.E.; Rahdar, A.; Pandey, S.; Jadoun, S.; Díez-Pascual, A.M. Curcumin delivery and co-delivery based on nanomaterials as an effective approach for cancer therapy. J. Drug. Deliv. Sci. Technol. 2022, 78, 103982. [Google Scholar] [CrossRef]
- Li, L.; Zhang, X.; Pi, C.; Yang, H.; Zheng, X.; Zhao, L.; Wei, Y. Review of Curcumin Physicochemical Targeting Delivery System. Int. J. Nanomed. 2020, 15, 9799–9821. [Google Scholar] [CrossRef]
- Zheng, B.; McClements, D.J. Formulation of More Efficacious Curcumin Delivery Systems Using Colloid Science: Enhanced Solubility, Stability, and Bioavailability. Molecules 2020, 25, 2791. [Google Scholar] [CrossRef] [PubMed]
- Kesharwani, P.; Banerjee, S.; Padhye, S.; Sarkar, F.H.; Iyer, A.K. Hyaluronic Acid Engineered Nanomicelles Loaded with 3,4-Difluorobenzylidene Curcumin for Targeted Killing of CD44+ Stem-Like Pancreatic Cancer Cells. Biomacromolecules 2015, 16, 3042–3053. [Google Scholar] [CrossRef] [PubMed]
- Datta, S.; Jutkova, A.; Šramkova, P.; Lenkavska, L.; Huntosova, V.; Chorva, D.; Miskovsky, P.; Jancura, D.; Kronek, J. Unravelling the Excellent Chemical Stability and Bioavailability of Solvent Responsive Curcumin-Loaded 2-Ethyl-2-oxazoline-grad-2-(4-dodecyloxyphenyl)-2-oxazoline Copolymer Nanoparticles for Drug Delivery. Biomacromolecules 2018, 19, 2459–2471. [Google Scholar] [CrossRef] [PubMed]
- Zatorska-Plachta, M.; Lazarski, G.; Maziarz, U.; Forys, A.; Trzebicka, B.; Wnuk, D.; Choluj, K.; Karewicz, A.; Michalik, M.; Jamroz, D.; et al. Encapsulation of Curcumin in Polystyrene-Based Nanoparticles Drug Loading Capacity and Cytotoxicity. ACS Omega 2021, 6, 12168–12178. [Google Scholar] [CrossRef] [PubMed]
- Bagheri, M.; Fens, M.H.; Kleijn, T.G.; Capomaccio, R.B.; Mehn, D.; Krawczyk, P.M.; Scutigliani, E.M.; Gurinov, A.; Baldus, M.; Kronenburg, N.C.H.V.; et al. In Vitro and In Vivo Studies on HPMA-Based Polymeric Micelles Loaded with Curcumin. Mol. Pharm. 2021, 18, 1247–1263. [Google Scholar] [CrossRef]
- Obeid, M.A.; Alsaadi, M.; Aljabali, A.A. Recent updates in curcumin delivery. J. Lipsome Res. 2023, 33, 53–64. [Google Scholar] [CrossRef] [PubMed]
- Feng, T.; Wei, Y.; Lee, R.J.; Zhao, L. Liposomal curcumin and its application in cancer. Int. J. Nanomed. 2017, 12, 6027–6044. [Google Scholar] [CrossRef]
- Lazar, A.N.; Mourtas, S.; Youssef, I.; Parizot, C.; Dauphin, A.; Delatour, B.; Antimisiaris, S.G.; Duyckaerts, C. Curcumin-conjugated nanoliposomes with high affinity for Ab deposits: Possible applications to Alzheimer disease. Nanomed 2013, 9, 712–721. [Google Scholar] [CrossRef]
- Mondal, G.; Barui, S.; Saha, S.; Chaudhuri, A. Tumor growth inhibition through targeting liposomally bound curcumin to tumor vasculature. J. Control Release 2013, 172, 832–840. [Google Scholar] [CrossRef]
- Arvapalli, D.M.; Sheardy, A.T.; Allado, K.; Chevva, H.; Yin, Z.; Wei, J. Design of Curcumin Loaded Carbon Nanodots Delivery System: Enhanced Bioavailability, Release Kinetics, and Anticancer Activity. ACS. Appl. Bio Mater. 2020, 3, 8776–8785. [Google Scholar] [CrossRef] [PubMed]
- Mathew, M.S.; Vinod, K.; Jayaram, P.S.; Jayasree, R.S.; Joseph, K. Improved Bioavailability of Curcumin in Gliadin-Protected Gold Quantum Cluster for Targeted Delivery. ACS Omega 2019, 4, 14169–14178. [Google Scholar] [CrossRef] [PubMed]
- Mal, A.S.P.R.; Valvi, S.K.; Srivastava, R.D.A.; Bandyopadhyaya, R. Noninvasive Preclinical Evaluation of Targeted Nanoparticles for the Delivery of Curcumin in Treating Pancreatic Cancer. ACS Appl. Bio Mater. 2020, 3, 4643–4654. [Google Scholar]
- Lawson, S.; Newport, K.; Pederniera, N.; Rownaghi, A.A.; Rezaei, F. Curcumin Delivery on Metal–Organic Frameworks: The Effect of the Metal Center on Pharmacokinetics within the M-MOF-74 Family. ACS Appl. Bio Mater. 2021, 4, 3423–3432. [Google Scholar] [CrossRef] [PubMed]
- Kotcherlakota, R.; Barui, A.K.; Prashar, S.; Fajardo, M.; Briones, D.; Rodriguez-Dieguez, A.; Patra, C.R.; Gomez-Ruiz, S. Curcumin loaded mesoporous silica: An effective drug delivery system for cancer treatment. Biomater. Sci. 2016, 4, 448–459. [Google Scholar] [CrossRef]
- Lin, Q.; Li, W.; Liu, D.; Zhao, M.; Zhu, X.; Li, W.; Wang, L.; Zheng, T.; Li, J. Porous Silicon Carrier Delivery System for Curcumin: Preparation, Characterization, and Cytotoxicity in Vitro. ACS Appl. Bio Mater. 2019, 2, 1041–1049. [Google Scholar] [CrossRef]
- Nasery, M.M.; Abadi, B.; Poormoghadam, D.; Zarrabi, A.; Keyhanvar, P.; Khanbabaei, H.; Ashrafizadeh, M.; Mohammadinejad, R.; Tavakol, S.; Sethi, G. Curcumin Delivery Mediated by Bio-Based Nanoparticles: A Review. Molecules 2020, 25, 689. [Google Scholar] [CrossRef]
- Wang, L.; Li, J.; Xiong, Y.; Wu, Y.; Yang, F.; Guo, Y.; Chen, Z.; Gao, L.; Deng, W. Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing. ACS Appl. Mater. Interfaces 2021, 13, 58329–58339. [Google Scholar] [CrossRef]
- Gao, C.; Chu, X.; Gong, W.; Zheng, J.; Xie, X.; Wang, Y.; Yang, M.; Li, Z.; Gao, C.; Yang, Y. Neuron tau-targeting biomimetic nanoparticles for curcumin delivery to delay progression of Alzheimer’s disease. J. Nanobiotechnol. 2020, 18, 71. [Google Scholar] [CrossRef]
- Yang, J.; Chen, X.; Wen, H.; Chen, Y.; Yu, Q.; Shen, M.; Xie, J. Curcumin-Loaded pH-Sensitive Biopolymer Hydrogels: Fabrication, Characterization, and Release Properties. ACS Food Sci. Technol. 2022, 2, 512–520. [Google Scholar] [CrossRef]
- Li, X.; He, Y.; Zhang, S.; Gu, Q.; McClements, D.J.; Chen, S.; Liu, X.; Liu, F. Lactoferrin-Based Ternary Composite Nanoparticles with Enhanced Dispersibility and Stability for Curcumin Delivery. ACS Appl. Mater. Interfaces 2023, 15, 18166–18181. [Google Scholar] [CrossRef]
- Wang, Z.; Zhang, R.X.; Zhang, C.; Dai, C.; Ju, X.; He, R. Fabrication of Stable and Self-Assembling Rapeseed Protein Nanogel for Hydrophobic Curcumin Delivery. J. Agric. Food Chem. 2019, 67, 887–894. [Google Scholar] [CrossRef] [PubMed]
- Milenkova, S.; Manolov, I.; Pilicheva, B.; Nikolova, M.; Marudova, M. Curcumin loaded casein submicron-sized gels as drug delivery systems. J. Phys. Conf. Ser. 2021, 1762, 012009. [Google Scholar] [CrossRef]
- Reeves, A.; Vinogradov, S.V.; Morrissey, P.; Chernin, M.; Ahmed, M.M. Curcumin-encapsulating Nanogels as an Effective Anticancer Formulation for Intracellular Uptake. Mol. Cell Pharmacol. 2015, 7, 25–40. [Google Scholar] [PubMed]
- Bisht, S.; Mizuma, M.; Feldmann, G.; Ottenhof, N.A.; Hong, S.; Pramanik, D.; Chenna, V.; Karikari, C.; Sharma, R.; Goggins, M.G.; et al. Systemic administration of polymeric nanoparticle-encapsulated curcumin (NanoCurc™) blocks tumor growth and metastases in preclinical models of pancreatic cancer. Mol. Cancer. Ther. 2010, 9, 2255–2264. [Google Scholar] [CrossRef] [PubMed]
- Ray, B.; Bisht, S.; Maitra, A.; Maitra, A.; Lahiri, D.K. Neuroprotective and Neurorescue Effects of a Novel Polymeric Nanoparticle Formulation of Curcumin (NanoCurc™) in the Neuronal Cell Culture and Animal Model: Implications for Alzheimer’s disease. J. Alzheimers. Dis. 2010, 23, 61–77. [Google Scholar] [CrossRef]
- Goncalves, C.; Pereira, P.; Schellenberg, P.; Coutinho, P.J.; Gama, F.M. Self-Assembled Dextrin Nanogel as Curcumin Delivery System. J. Biomater. Nanobiotechnol. 2012, 3, 178–184. [Google Scholar] [CrossRef]
- Luckanagul, J.A.; Pitakchatwong, C.; Bhuket, P.R.N.; Muangnoi, C.; Rojsitthisak, P.; Chirachanchai, S.; Wang, Q.; Rojsitthisak, P. Chitosan-based polymer hybrids for thermo-responsive nanogel delivery of curcumin. Carbohydr. Polym. 2018, 181, 1119–1127. [Google Scholar] [CrossRef]
- Dinari, A.; Abdollahi, M.; Sadeghizadeh, M. Design and fabrication of dual responsive lignin-based nanogel via “grafting from” atom transfer radical polymerization for curcumin loading and release. Sci. Rep. 2021, 11, 1962. [Google Scholar] [CrossRef] [PubMed]
- Santhamoorthy, M.; Kim, S. Dual pH- and Thermo-Sensitive Poly(N-Isopropylacrylamide-co-Allylamine) Nanogels for Curcumin Delivery: Swelling–Deswelling Behavior and Phase Transition Mechanism. Gels 2023, 9, 536. [Google Scholar] [CrossRef]
- Howaili, F.; Özliseli, E.; Kucukturkmen, B.; Razavi, S.M.; Sadeghizadeh, M.; Rosenholm, J.M. Stimuli-Responsive, Plasmonic Nanogel for Dual Delivery of Curcumin and Photothermal Therapy for Cancer Treatment. Front. Chem. 2021, 8, 602941. [Google Scholar] [CrossRef] [PubMed]
- Oh, J.K.; Lee, D.I.; Park, J.M. Biopolymer-based microgels/nanogels for drug delivery applications. Prog. Polym. Sci. 2009, 34, 1261–1282. [Google Scholar] [CrossRef]
- Yin, Y.; Hu, B.; Yuan, X.; Cai, L.; Gao, H.; Yang, Q. Nanogel: A Versatile Nano-Delivery System for Biomedical Applications. Pharmaceutics 2020, 12, 290. [Google Scholar] [CrossRef] [PubMed]
- Preman, N.K.; Jain, S.; Johnson, R.P. “Smart” Polymer Nanogels as Pharmaceutical Carriers: A Versatile Platform for Programmed Delivery and Diagnostics. ACS Omega 2021, 6, 5075–5090. [Google Scholar] [CrossRef]
- Wu, W.; Shen, J.; Banerjee, P.; Zhou, S. Water-dispersible multifunctional hybrid nanogels for combined curcumin and photothermal therapy. Biomaterials 2011, 32, 598–609. [Google Scholar] [CrossRef]
- Badi, N. Non-linear PEG-based thermoresponsive polymer systems. Prog. Polym. Sci. 2017, 66, 54–79. [Google Scholar] [CrossRef]
- Lutz, J.F.; Akdemir, Ö.; Hoth, A. Point by point comparison of two thermosensitive polymers exhibiting a similar LCST: Is the age of poly(NIPAM) over. J. Am. Chem. Soc. 2006, 128, 13046–13047. [Google Scholar] [CrossRef]
- Liu, M.; Leroux, J.C.; Gauthier, M.A. Conformation-function relationships for the comb-shaped polymer pOEGMA. Prog. Polym. Sci. 2015, 48, 111–121. [Google Scholar] [CrossRef]
- Lutz, J.F. Polymerization of oligo(ethylene glycol) (meth)acrylates: Toward new generations of smart biocompatible materials. J. Polym. Sci. Part A Polym. Chem. 2008, 46, 3459–3470. [Google Scholar] [CrossRef]
- Hu, Z.; Cai, T.; Chi, C. Thermoresponsive oligo(ethylene glycol)-methacrylatebased polymers and microgels. Soft Matter 2010, 6, 2115–2123. [Google Scholar] [CrossRef]
- Lutz, J.F. Thermo-switchable materials prepared using the OEGMA-platform. Adv. Mater. 2011, 23, 2237–2243. [Google Scholar] [CrossRef]
- Ghosh, S.; GhoshMitra, S.; Cai, T.; Diercks, D.R.; Mills, N.C.; Hynds, D.A.L. Alternating Magnetic Field Controlled, Multifunctional Nano-Reservoirs: Intracellular Uptake and Improved Biocompatibility. Nanoscale Res. Lett. 2010, 5, 195. [Google Scholar] [CrossRef] [PubMed]
- Hayes, J.L.; Ingram, L.L.; Strom, L.B.; Roton, L.M.; Boyette, M.W.; Walsh, M.T. USDA Forest Service, Southern Forest Experiment Station, General Technical Report SO-104. In Proceedings of the 4th Southern Station Chemical Sciences Meeting, Starkville, MS, USA, 1–2 February 1994; Vozzo, J.A., Ed.; 2004; pp. 69–80. [Google Scholar]
- Fiege, H.; Voges, H.W.; Hamamoto, T.; Umemura, S.; Iwata, T.; Miki, H.; Fujita, Y.; Buysch, H.J.; Garbe, D.; Paulus, W. Phenol Derivatives. In Ullmann’s Encyclopedia of Industrial Chemistry; Wiley-VCH: Weinheim, Germany, 2002; pp. 521–582. [Google Scholar]
- Chi, C.; Cai, T.; Hu, Z. Oligo(ethylene glycol)-Based Thermoresponsive Core−Shell Microgels. Langmuir 2009, 25, 3814–3819. [Google Scholar] [CrossRef]
- Berndt, I.; Pedersen, J.S.; Lindner, P.; Richtering, W. Influence of Shell Thickness and Cross-Link Density on the Structure of Temperature-Sensitive Poly-N-Isopropylacrylamide−Poly-N-Isopropylmethacrylamide Core−Shell Microgels Investigated by Small-Angle Neutron Scattering. Langmuir 2006, 22, 459–468. [Google Scholar] [CrossRef] [PubMed]
- Zhou, T.; Wu, W.; Zhou, S. Engineering Oligo(ethylene glycol)-Based Thermo-sensitive Microgels for Drug Delivery Applications. Polymer 2010, 51, 3926–3933. [Google Scholar] [CrossRef]
- Schneider, C.; Gordon, O.N.; Edwards, R.L.; Luis, P.B. Degradation of curcumin: From mechanism to biological implications. J. Agric. Food Chem. 2015, 63, 7606–7614. [Google Scholar] [CrossRef] [PubMed]
- Wang, Y.; Pan, M.; Cheng, A.; Lin, L.; Ho, Y.; Hsieh, C.; Lin, J. Stability of curcumin in buffer solutions and characterization of its degradation products. J. Pharm. Biomed. Anal. 1997, 15, 1867–1876. [Google Scholar] [CrossRef]
- Mondal, S.; Ghosh, S.; Moulik, S.P. Stability of curcumin in different solvent and solution media: UV–visible and steady-state fluorescence spectral study. J. Photochem. Photobiol. B 2016, 158, 212–218. [Google Scholar] [CrossRef]
- Iversena, T.-G.; Skotlanda, T.; Sandvig, K. Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies. Nano Today 2011, 6, 176–185. [Google Scholar] [CrossRef]
- Manzanares, D.; Ceña, V. Endocytosis: The Nanoparticle and Submicron Nanocompounds Gateway into the Cell. Pharmaceutics 2020, 12, 371. [Google Scholar] [CrossRef]
- Wu, W.; Shen, J.; Li, Y.; Zhu, H.; Banerjee, P.; Zhou, S. Specific glucose-to-SPR signal transduction at physiological pH by molecularly imprinted responsive hybrid microgels. Biomaterials 2012, 33, 7115–7125. [Google Scholar] [CrossRef] [PubMed]
- Chu, B. Laser Light Scattering, 2nd ed.; Academic Press: New York, NY, USA, 1991. [Google Scholar]
- Berne, B.J.; Pecora, R. Dynamic Light Scattering; Plenum Press: New York, NY, USA, 1976. [Google Scholar]
Sample | Core Solution (mmol) | Shell Solution (mmol) | Rh (nm) | ||
---|---|---|---|---|---|
2-Vinylanisole | MEO2MA | MEO5MA | PEGDMA | ||
PVAS core nanogel | 0.594 | –– | –– | –– | 70 |
PEG shell nanogel | –– | 0.75 | 1.50 | 2.26 × 10−2 | –– |
VEM1 | 0.594 | 0.20 | 0.40 | 0.60 × 10−2 | 101 |
VEM2 | 0.594 | 0.25 | 0.50 | 0.75 × 10−2 | 110 |
VEM3 | 0.594 | 0.50 | 1.00 | 1.51 × 10−2 | 138 |
VEM4 | 0.594 | 0.75 | 1.50 | 2.26 × 10−2 | 165 |
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Shen, J.; Zhang, J.; Wu, W.; Banerjee, P.; Zhou, S. Biocompatible Anisole-Nonlinear PEG Core–Shell Nanogels for High Loading Capacity, Excellent Stability, and Controlled Release of Curcumin. Gels 2023, 9, 762. https://doi.org/10.3390/gels9090762
Shen J, Zhang J, Wu W, Banerjee P, Zhou S. Biocompatible Anisole-Nonlinear PEG Core–Shell Nanogels for High Loading Capacity, Excellent Stability, and Controlled Release of Curcumin. Gels. 2023; 9(9):762. https://doi.org/10.3390/gels9090762
Chicago/Turabian StyleShen, Jing, Jiangtao Zhang, Weitai Wu, Probal Banerjee, and Shuiqin Zhou. 2023. "Biocompatible Anisole-Nonlinear PEG Core–Shell Nanogels for High Loading Capacity, Excellent Stability, and Controlled Release of Curcumin" Gels 9, no. 9: 762. https://doi.org/10.3390/gels9090762