Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Inoculum
2.2. Microfluidic Device Model and Imaging
2.3. Biofilm Formation
2.4. Human Neutrophil Collection
2.5. Scanning Electron Microscopy
2.6. Measurement of ROS
2.7. Sytox Green Assays
2.8. Fluorescent Imaging
2.9. Statistics
3. Results
3.1. Biofilms Produced by C. albicans Clinical Isolates Differ in Architecture and Thickness
3.2. C. albicans Biofilms Variably Inhibit ROS Production by Neutrophils
3.3. C. albicans Biofilms Uniformly Inhibit the Formation of NETs
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kernien, J.F.; Johnson, C.J.; Nett, J.E. Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms. J. Fungi 2017, 3, 49. https://doi.org/10.3390/jof3030049
Kernien JF, Johnson CJ, Nett JE. Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms. Journal of Fungi. 2017; 3(3):49. https://doi.org/10.3390/jof3030049
Chicago/Turabian StyleKernien, John F., Chad J. Johnson, and Jeniel E. Nett. 2017. "Conserved Inhibition of Neutrophil Extracellular Trap Release by Clinical Candida albicans Biofilms" Journal of Fungi 3, no. 3: 49. https://doi.org/10.3390/jof3030049