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J. Fungi 2017, 3(3), 37; doi:10.3390/jof3030037

Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis

1
Dipartimento diScienze Veterinarie, Università di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
2
Ecole nationale Vétérinaire d’Alfort, Dynamyc Research Group, EnvA, Université, Maisons-Alfort 94700, France
3
Public Health England Mycology Reference Laboratory, Kingsdown, Bristol BS2 8EL, UK
*
Author to whom correspondence should be addressed.
Received: 6 May 2017 / Revised: 21 June 2017 / Accepted: 29 June 2017 / Published: 5 July 2017
(This article belongs to the Special Issue Antifungal Susceptibility Testing)
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Abstract

Reference methods for antifungal susceptibility testing of yeasts have been developed by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antibiotic Susceptibility Testing (EUCAST). These methods are intended to test the main pathogenic yeasts that cause invasive infections, namely Candida spp. and Cryptococcus neoformans, while testing other yeast species introduces several additional problems in standardization not addressed by these reference procedures. As a consequence, a number of procedures have been employed in the literature to test the antifungal susceptibility of Malassezia pachydermatis. This has resulted in conflicting results. The aim of the present study is to review the procedures and the technical parameters (growth media, inoculum preparation, temperature and length of incubation, method of reading) employed for susceptibility testing of M. pachydermatis, and when possible, to propose recommendations for or against their use. Such information may be useful for the future development of a reference assay. View Full-Text
Keywords: Malassezia pachydermatis; susceptibility testing; broth microdilution; disk diffusion; minimum inhibitory concentration (MIC) Malassezia pachydermatis; susceptibility testing; broth microdilution; disk diffusion; minimum inhibitory concentration (MIC)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Peano, A.; Pasquetti, M.; Tizzani, P.; Chiavassa, E.; Guillot, J.; Johnson, E. Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis. J. Fungi 2017, 3, 37.

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