The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
AbstractThis paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding strategies, was carried out. The feedstock comprised volatile fatty acids, which were applied as single carbon sources, as mixtures, or in series, using a fed-batch feed-on-demand controlled bioprocess. A dynamic trend in active biomass growth as well as polymer composition was observed. The observations were consistent over replicate accumulations. Metabolic flux analysis (MFA) was used to investigate metabolic activity through time. It was concluded that carbon flux, and consequently copolymer composition, could be linked with how reducing equivalents are generated. View Full-Text
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Montano-Herrera, L.; Laycock, B.; Werker, A.; Pratt, S. The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents. Bioengineering 2017, 4, 20.
Montano-Herrera L, Laycock B, Werker A, Pratt S. The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents. Bioengineering. 2017; 4(1):20.Chicago/Turabian Style
Montano-Herrera, Liliana; Laycock, Bronwyn; Werker, Alan; Pratt, Steven. 2017. "The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents." Bioengineering 4, no. 1: 20.
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