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Bioengineering 2016, 3(1), 3; doi:10.3390/bioengineering3010003

13C-Metabolic Flux Analysis: An Accurate Approach to Demystify Microbial Metabolism for Biochemical Production

Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Author to whom correspondence should be addressed.
Academic Editors: Mark Blenner and Michael D. Lynch
Received: 1 October 2015 / Revised: 10 December 2015 / Accepted: 18 December 2015 / Published: 25 December 2015
(This article belongs to the Special Issue Metabolic Engineering)
View Full-Text   |   Download PDF [4124 KB, uploaded 25 December 2015]   |  


Metabolic engineering of various industrial microorganisms to produce chemicals, fuels, and drugs has raised interest since it is environmentally friendly, sustainable, and independent of nonrenewable resources. However, microbial metabolism is so complex that only a few metabolic engineering efforts have been able to achieve a satisfactory yield, titer or productivity of the target chemicals for industrial commercialization. In order to overcome this challenge, 13C Metabolic Flux Analysis (13C-MFA) has been continuously developed and widely applied to rigorously investigate cell metabolism and quantify the carbon flux distribution in central metabolic pathways. In the past decade, many 13C-MFA studies have been performed in academic labs and biotechnology industries to pinpoint key issues related to microbe-based chemical production. Insightful information about the metabolic rewiring has been provided to guide the development of the appropriate metabolic engineering strategies for improving the biochemical production. In this review, we will introduce the basics of 13C-MFA and illustrate how 13C-MFA has been applied via integration with metabolic engineering to identify and tackle the rate-limiting steps in biochemical production for various host microorganisms View Full-Text
Keywords: Bottleneck; isotope; cofactor imbalance; cell metabolism; synthetic biology; biofuels Bottleneck; isotope; cofactor imbalance; cell metabolism; synthetic biology; biofuels

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Guo, W.; Sheng, J.; Feng, X. 13C-Metabolic Flux Analysis: An Accurate Approach to Demystify Microbial Metabolism for Biochemical Production. Bioengineering 2016, 3, 3.

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