Next Article in Journal
Characterisation of Bone Beneficial Components from Australian Wallaby Bone
Next Article in Special Issue
Antidermatophytic Activity of the Fruticose Lichen Usnea orientalis
Previous Article in Journal
Why We Need Minimum Basic Requirements in Science for Acupuncture Education
Previous Article in Special Issue
Antimicrobial, Cytotoxic, Phytotoxic and Antioxidant Potential of Heliotropium strigosum Willd.
Article Menu

Export Article

Open AccessArticle
Medicines 2016, 3(3), 22;

Cytotoxic and Antimicrobial Constituents from the Essential Oil of Lippia alba (Verbenaceae)

Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, SP, Brazil
Disciplina de Biologia Celular, Departamento de Micro, Imuno e Parasitologia, Universidade Federal de São Paulo, Sao Paulo 04021-001, SP, Brazil
Instituto de Química, Universidade Federal de Alfenas, Alfenas 37130-000, MG, Brazil
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo Andre 09210-180, SP, Brazil
Authors to whom correspondence should be addressed.
Academic Editor: James D. Adams
Received: 30 June 2016 / Revised: 8 August 2016 / Accepted: 9 August 2016 / Published: 12 August 2016
(This article belongs to the Special Issue Plant Medicines for Clinical Trial)
View Full-Text   |   Download PDF [448 KB, uploaded 15 August 2016]   |  


Backgroud: Lippia alba (Verbenaceae) is a plant widely used in folk medicine to treat various diseases. The present work deals with the chemical composition of the crude essential oil extracted from leaves of L. alba and evaluation of its antimicrobial and cytotoxic activities. Methods: Leaves of L. alba were extracted by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) as well as by nuclear magnetic resonance (NMR) spectroscopy. Cytotoxic and antimicrobial activities of crude essential oil were evaluated in vitro using MTT and broth microdilution assays, respectively. Results: Chemical analysis afforded the identification of 39 substances corresponding to 99.45% of the total oil composition. Concerning the main compounds, monoterpenes nerol/geraniol and citral correspond to approximately 50% of crude oil. The cytotoxic activity of obtained essential oil against several tumor cell lines showed IC50 values ranging from 45 to 64 µg/mL for B16F10Nex2 (murine melanoma) and A549 (human lung adenocarcinoma). In the antimicrobial assay, was observed that all tested yeast strains, except C. albicans, were sensitive to crude essential oil. MIC values were two to four-folds lower than those determined to bacterial strains. Conclusion: Analysis of chemical composition of essential oils from leaves of L. alba suggested a new chemotype nerol/geraniol and citral. Based in biological evidences, a possible application for studied oil as an antifungal in medicine, as well as in agriculture, is described. View Full-Text
Keywords: Lippia alba; essential oil; cytotoxic activity; antimicrobial activity Lippia alba; essential oil; cytotoxic activity; antimicrobial activity

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Santos, N.O.; Pascon, R.C.; Vallim, M.A.; Figueiredo, C.R.; Soares, M.G.; Lago, J.H.G.; Sartorelli, P. Cytotoxic and Antimicrobial Constituents from the Essential Oil of Lippia alba (Verbenaceae). Medicines 2016, 3, 22.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Medicines EISSN 2305-6320 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top