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Foods 2018, 7(1), 8; doi:10.3390/foods7010008

Absorption and Metabolism of Phenolics from Digests of Polyphenol-Rich Potato Extracts Using the Caco-2/HepG2 Co-Culture System

1
School of Human Nutrition, McGill University, 21111 Lakeshore, Ste. Anne de Bellevue, Montreal, QC H9X3V9, Canada
2
Chemistry Department, Université du Québec à Montréal, 2101 Jeanne-Mance, Montreal, QC H2X2J6, Canada
3
Department of Biomedical Engineering, Duff Medical Building, 3775 Rue University, Montreal, QC H3A2B4, Canada
*
Author to whom correspondence should be addressed.
Received: 1 December 2017 / Revised: 23 December 2017 / Accepted: 9 January 2018 / Published: 12 January 2018
(This article belongs to the Section Food Function and Nutrition)
View Full-Text   |   Download PDF [240 KB, uploaded 12 January 2018]

Abstract

The bioactivity of dietary polyphenols depends upon gastrointestinal and hepatic metabolism of secondary microbial phenolic metabolites generated via colonic microbiota-mediated biotransformation. A polyphenol-rich potato extract (PRPE) containing chlorogenic, caffeic, and ferulic acids and rutin was digested in a dynamic multi-reactor gastrointestinal simulator of the human intestinal microbial ecosystem (GI model). Simulated digestion showed extensive degradation of the parent compounds and the generation of microbial phenolic metabolites. To characterize the transport and metabolism of microbial phenolic metabolites following digestion, a co-culture of intestinal Caco-2 and hepatic HepG2 cells was exposed to the PRPE-derived digests obtained from the colonic vessels. Following a 2 h incubation of the digesta with the Caco-2/HepG2 co-cultures, approximately 10–15% of ferulic, dihydrocaffeic, and dihydroferulic acids and 3–5% of 3-hydroxybenzoic, 3-hydroxyphenylpropionic, and coumaric acids were observed in the basolateral side, whereas 3-hydroxyphenylacetic acid, phenylpropanoic acid, and cinnamic acid were not detected. Subsequent HepG2 cellular metabolism led to major increases in ferulic, dihydrocaffeic, 3-hydroxyphenylpropionic, and coumaric acids ranging from 160–370%. These findings highlight the importance of hepatic metabolism towards the generation of secondary metabolites of polyphenols despite low selective Caco-2 cellular uptake of microbial phenolic metabolites. View Full-Text
Keywords: in vitro digestion; Caco-2/HepG2 co-culture; potato; chlorogenic acid; ferulic acid; caffeic acid; rutin in vitro digestion; Caco-2/HepG2 co-culture; potato; chlorogenic acid; ferulic acid; caffeic acid; rutin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sadeghi Ekbatan, S.; Iskandar, M.M.; Sleno, L.; Sabally, K.; Khairallah, J.; Prakash, S.; Kubow, S. Absorption and Metabolism of Phenolics from Digests of Polyphenol-Rich Potato Extracts Using the Caco-2/HepG2 Co-Culture System. Foods 2018, 7, 8.

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