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Photonics 2017, 4(3), 40; doi:10.3390/photonics4030040

Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM + FLIM)

1
Photonics Group, Physics Department, Imperial College London, South Kensington, London SW7 2AZ, UK
2
National Heart and Lung Institute, Imperial College London, South Kensington, London SW7 2AZ, UK
3
Centre for Histopathology, Imperial College London, Du Cane Road, London SW7 2AZ, UK
*
Author to whom correspondence should be addressed.
Received: 16 May 2017 / Revised: 30 June 2017 / Accepted: 30 June 2017 / Published: 7 July 2017
(This article belongs to the Special Issue Superresolution Optical Microscopy)
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Abstract

We present a new microscope integrating super-resolved imaging using structured illumination microscopy (SIM) with wide-field optically sectioned fluorescence lifetime imaging (FLIM) to provide optical mapping of molecular function and its correlation with biological nanostructure below the conventional diffraction limit. We illustrate this SIM + FLIM capability to map FRET readouts applied to the aggregation of discoidin domain receptor 1 (DDR1) in Cos 7 cells following ligand stimulation and to the compaction of DNA during the cell cycle. View Full-Text
Keywords: fluorescence lifetime microscopy; structured illumination microscopy; Förster energy transfer; collagen receptor; DNA fluorescence lifetime microscopy; structured illumination microscopy; Förster energy transfer; collagen receptor; DNA
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Görlitz, F.; Corcoran, D.S.; Garcia Castano, E.A.; Leitinger, B.; Neil, M.A.A.; Dunsby, C.; French, P.M.W. Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM + FLIM). Photonics 2017, 4, 40.

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