Momilactones A and B: Optimization of Yields from Isolation and Purification
AbstractMomilactones A and B (MA and MB, respectively) are phytoalexins and plant growth inhibitors available in rice husks. However, to date, the isolation and purification of the two compounds have been complicated, laborious, and less effective. The present study was conducted to establish a protocol to simplify and optimize quantities of MA and MB by combinations of ethyl acetate (EtOAc), distilled water, methanol (MeOH), temperature, and pressure for extractions. At a temperature of 100 °C, combined with EtOAc and MeOH 100%, MA and MB were enriched in greater quantities than non-treated rice husks, of which MB was much augmented than MA. The EtOAc extract obtained from samples dried at 100 °C for 1 h, then placed in MeOH 100% for 1 week, provided maximum yields of MA [58.76 µg/g dry weight (DW)] and MB (104.43 µg/g DW). The use of pressure effectively enhanced yields of MA (17.90–26.26 µg/g DW) and MB (40.78–71.0 µg/g DW). The actual purified amounts of MA and MB increased by 5 and 15 folds, respectively. The use of either sole distilled water or MeOH ≤ 50% at any temperature did not successfully isolate both MA and MB. The yield optimization aids to easier and more productive purification of the two compounds, and thus extends researches on biological activities of MA and MB, including pharmaceutical and medicinal properties. View Full-Text
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Ngoc Minh, T.; Xuan, T.D.; Ahmad, A.; Abdelghany Elzaawely, A.; Teschke, R.; Van, T.M. Momilactones A and B: Optimization of Yields from Isolation and Purification. Separations 2018, 5, 28.
Ngoc Minh T, Xuan TD, Ahmad A, Abdelghany Elzaawely A, Teschke R, Van TM. Momilactones A and B: Optimization of Yields from Isolation and Purification. Separations. 2018; 5(2):28.Chicago/Turabian Style
Ngoc Minh, Truong; Xuan, Tran D.; Ahmad, Ateeque; Abdelghany Elzaawely, Abdelnaser; Teschke, Rolf; Van, Truong M. 2018. "Momilactones A and B: Optimization of Yields from Isolation and Purification." Separations 5, no. 2: 28.
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