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Separations 2018, 5(2), 28; https://doi.org/10.3390/separations5020028

Momilactones A and B: Optimization of Yields from Isolation and Purification

1
Graduate school for International Development and Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan
2
Process Chemistry and Technology Department, Central Institute of Medicinal and Aromatic Plants, Lucknow 226016, India
3
Department of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31527, Egypt
4
Department of Internal Medicine II, Division of Gastroenterology and Hepatology, Klinikum Hanau, D-63450 Hanau, Germany
5
Teaching Hospital of the Medical Faculty, Goethe University Frankfurt, D-60590 Frankfurt, Germany
*
Author to whom correspondence should be addressed.
Received: 21 March 2018 / Revised: 19 April 2018 / Accepted: 7 May 2018 / Published: 14 May 2018
(This article belongs to the Special Issue Analytical Tools for Food Analysis)
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Abstract

Momilactones A and B (MA and MB, respectively) are phytoalexins and plant growth inhibitors available in rice husks. However, to date, the isolation and purification of the two compounds have been complicated, laborious, and less effective. The present study was conducted to establish a protocol to simplify and optimize quantities of MA and MB by combinations of ethyl acetate (EtOAc), distilled water, methanol (MeOH), temperature, and pressure for extractions. At a temperature of 100 °C, combined with EtOAc and MeOH 100%, MA and MB were enriched in greater quantities than non-treated rice husks, of which MB was much augmented than MA. The EtOAc extract obtained from samples dried at 100 °C for 1 h, then placed in MeOH 100% for 1 week, provided maximum yields of MA [58.76 µg/g dry weight (DW)] and MB (104.43 µg/g DW). The use of pressure effectively enhanced yields of MA (17.90–26.26 µg/g DW) and MB (40.78–71.0 µg/g DW). The actual purified amounts of MA and MB increased by 5 and 15 folds, respectively. The use of either sole distilled water or MeOH ≤ 50% at any temperature did not successfully isolate both MA and MB. The yield optimization aids to easier and more productive purification of the two compounds, and thus extends researches on biological activities of MA and MB, including pharmaceutical and medicinal properties. View Full-Text
Keywords: momilactone A; momilactone B; column chromatography; yield optimization; temperature; pressure; extracting solvents momilactone A; momilactone B; column chromatography; yield optimization; temperature; pressure; extracting solvents
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Ngoc Minh, T.; Xuan, T.D.; Ahmad, A.; Abdelghany Elzaawely, A.; Teschke, R.; Van, T.M. Momilactones A and B: Optimization of Yields from Isolation and Purification. Separations 2018, 5, 28.

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