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Separations 2018, 5(2), 29; https://doi.org/10.3390/separations5020029

Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution

1
Institute of Biomedical Chemistry, Pogodinskaya st., 10, Moscow 119121, Russia
2
Foundation of Perspective Technologies and Novations, Moscow 115682, Russia
3
Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow 125412, Russia
4
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk 220141, Belarus
*
Author to whom correspondence should be addressed.
Received: 27 December 2017 / Revised: 29 March 2018 / Accepted: 4 May 2018 / Published: 21 May 2018
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Abstract

It is known that a charge is generated in water and protein solutions upon their motion; this phenomenon is also observed in analytical systems for atomic force microscopy (AFM)-based fishing. At that, the efficiency of protein fishing correlates with the value of charge, generated upon the motion of the analyzed solution. Earlier, we demonstrated that a pulsed electric field can well be used for the enhancement of the efficiency of AFM-based fishing of low-abundant protein. In this paper, we have demonstrated the influence of a pulsed electric field on the stimulation of the electric charge generation in a solution of low-abundant proteins observed in the injector part of an AFM-based fishing system at 23 °C and 38 °C. Taking this effect into account is important for the development of novel highly sensitive flow-based diagnostic systems, as well as for the development of models describing the influence of a pulsed electric field on pathological processes in the body, hemodynamics, and physicochemical properties of solutions. View Full-Text
Keywords: analytical flow-based systems; flowing protein solution; charge accumulation analytical flow-based systems; flowing protein solution; charge accumulation
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Ivanov, Y.D.; Kozlov, A.F.; Galiullin, R.A.; Tatur, V.Y.; Ziborov, V.S.; Usanov, S.A.; Pleshakova, T.O. Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution. Separations 2018, 5, 29.

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