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Separations 2018, 5(1), 3; https://doi.org/10.3390/separations5010003

Chiral Recognition by DNA-Immobilized TLC Plate

Department of Chemistry, Faculty of Science, Okayama University of Science, Ridaicho 1-1, Kita-ku, Okayama 700-0005, Japan
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Received: 8 November 2017 / Revised: 20 December 2017 / Accepted: 22 December 2017 / Published: 26 December 2017
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Abstract

DNA-immobilized silica gel TLC plate (DNA-TLC plate) by coating a mixture of double-stranded DNA and silica gel on a glass plate was stable in common organic solvents, such as alcohol, acetone, chloroform, and ethyl acetate. DNA-TLC plate showed different Rf values for the L- and D-form acidic amino acids, such as aspartic acid and glutamic acid. The chiral recognition of an acidic amino acid by the DNA-TLC was related to the interaction between the nucleic acid base, particularly guanine (G) and cytosine (C), and the amino acid. Furthermore, the DNA-TLC plate indicated the recognition of a chiral metal complex, such as the tris(1,10-phenanthroline)ruthenium(II) ([Ru(phen)3]2+) salt, which has Λ- and Δ-forms. Therefore, the double-stranded DNA-TLC plate may have the potential to be utilized as a chiral separation material for amino acids, peptides, and a metal complex with a chiral property. View Full-Text
Keywords: chiral recognition; DNA; amino acid; metal complex; thin layer chromatography; organic-inorganic hybrid material chiral recognition; DNA; amino acid; metal complex; thin layer chromatography; organic-inorganic hybrid material
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Yamada, M.; Inoue, M. Chiral Recognition by DNA-Immobilized TLC Plate. Separations 2018, 5, 3.

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