Next Article in Journal
Evaluation of Ion Exchange and Sorbing Materials for Their Adsorption/Desorption Performane towards Anthocyanins, Total Phenolics, and Sugars from a Grape Pomace Extract
Next Article in Special Issue
Separation of Small DNAs by Gel Electrophoresis in a Fused Silica Capillary Coated with a Negatively Charged Copolymer
Previous Article in Journal
Recent Advances in Extraction and Stirring Integrated Techniques
Article Menu

Export Article

Open AccessArticle
Separations 2017, 4(1), 8; doi:10.3390/separations4010008

Comparison of Miniaturized and Conventional Asymmetrical Flow Field-Flow Fractionation (AF4) Channels for Nanoparticle Separations

1
Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Street 11, D-12489 Berlin, Germany
2
Postnova Analytics GmbH, Max-Planck-Street 14, D-86899 Landsberg am Lech, Germany
*
Author to whom correspondence should be addressed.
Received: 2 December 2016 / Revised: 19 December 2016 / Accepted: 8 March 2017 / Published: 18 March 2017
(This article belongs to the Special Issue Size Separation Techniques)
View Full-Text   |   Download PDF [1760 KB, uploaded 20 March 2017]   |  

Abstract

The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP) using Asymmetrical Flow Field-Flow Fractionation (AF4) was investigated and compared with a conventional AF4 system. To develop standard separation methods, experimental parameters like cross flow, gradient profile and injection time were varied and optimized. Corresponding chromatographic parameters were calculated and compared. Our results indicate that the chromatographic resolution in the miniaturized channel is lower, whereas significantly shorter analyses time and less solvent consumption were obtained. Moreover, the limit of detection (LOD) and limit of quantification (LOQ) obtained from hyphenation with a UV-detector are obviously lower than in a conventional channel, which makes the miniaturized channel interesting for trace analysis. View Full-Text
Keywords: nanoparticles; field-flow fractionation; miniaturized channel; detection limit; resolution nanoparticles; field-flow fractionation; miniaturized channel; detection limit; resolution
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

You, Z.; Meier, F.; Weidner, S. Comparison of Miniaturized and Conventional Asymmetrical Flow Field-Flow Fractionation (AF4) Channels for Nanoparticle Separations. Separations 2017, 4, 8.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Separations EISSN 2297-8739 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top