Next Article in Journal
Review: The Application of Liquid Chromatography Electrochemical Detection for the Determination of Drugs of Abuse
Next Article in Special Issue
Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement
Previous Article in Journal
Achieving a Near-Theoretical Maximum in Peak Capacity Gain for the Forensic Analysis of Ignitable Liquids Using GC×GC-TOFMS
Article Menu

Export Article

Open AccessFeature PaperReview
Separations 2016, 3(3), 27; doi:10.3390/separations3030027

Chromatographic Studies of Protein-Based Chiral Separations

Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA
*
Author to whom correspondence should be addressed.
Academic Editor: W John Lough
Received: 12 June 2016 / Revised: 21 July 2016 / Accepted: 12 August 2016 / Published: 5 September 2016
(This article belongs to the Special Issue Advance in Chiral Chromatography)
View Full-Text   |   Download PDF [2775 KB, uploaded 5 September 2016]   |  

Abstract

The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations. View Full-Text
Keywords: chiral high-performance liquid chromatography (HPLC); protein-based chiral stationary phases; chiral recognition; chromatographic studies of drug-protein interactions; frontal analysis; zonal elution chiral high-performance liquid chromatography (HPLC); protein-based chiral stationary phases; chiral recognition; chromatographic studies of drug-protein interactions; frontal analysis; zonal elution
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Bi, C.; Zheng, X.; Azaria, S.; Beeram, S.; Li, Z.; Hage, D.S. Chromatographic Studies of Protein-Based Chiral Separations. Separations 2016, 3, 27.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Separations EISSN 2297-8739 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top