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Separations 2016, 3(3), 23; doi:10.3390/separations3030023

Gram-Scale Purification of Dihydrorobinetin from Robinia pseudoacacia L. Wood by Centrifugal Partition Chromatography

1
Univ. Orléans, CNRS, ICOA, UMR 7311, F-45067 Orléans, France
2
AGPF, INRA, 45075 Orléans, France
3
GénoBois Wood Analysis Platform, INRA, 45075 Orléans, France
4
IUT d’Orléans, Univ. Orléans, F-45067 Orléans, France
5
Alban Muller International, 28630 Fontenay-sur-Eure, France
*
Author to whom correspondence should be addressed.
Academic Editor: Alain Berthod
Received: 16 June 2016 / Revised: 25 July 2016 / Accepted: 28 July 2016 / Published: 11 August 2016
(This article belongs to the Special Issue Countercurrent Chromatography)
View Full-Text   |   Download PDF [2349 KB, uploaded 11 August 2016]   |  

Abstract

Robinia pseudoacacia L. is a tree widely dispersed in France that is characterized by good growth rates and important biomass production, which produces wood with very high natural durability used for outdoor fence posts, timber, and barrels to age vinegars and wines. Its mature heartwood presents high resistance against wood fungi decay and contains two main flavonoid extractives, dihydrorobinetin—the most abundant—and robinetin that present interesting biological activities. The aim of the present study was to optimize a procedure allowing an important recovery of purified dihydrorobinetin from R. pseudoacacia wood, representing an interesting sustainable, local, highly available, and, consequently, economical source of bioactive components. The extraction of dihydrorobinetin was first optimized by evaluating the influence of various extraction parameters such as temperature, extraction time, solvent nature, and wood/solvent mass ratio to obtain an efficient, safe, and low cost extraction. Then, dihydrorobinetin was purified over 95% using centrifugal partition chromatography (CPC). CPC purification was first developed on a small volume column with low amounts of injected extract, then scaled-up on a 200 mL column with higher sample loading capacity in order to purify more than 1.3 g of dihydrorobinetin in one run. View Full-Text
Keywords: centrifugal partition chromatography; mass spectrometry; flavonoids; Robinia pseudoacacia wood extracts; dihydrorobinetin centrifugal partition chromatography; mass spectrometry; flavonoids; Robinia pseudoacacia wood extracts; dihydrorobinetin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Destandau, E.; Charpentier, J.-P.; Bostyn, S.; Zubrzycki, S.; Serrano, V.; Seigneuret, J.-M.; Breton, C. Gram-Scale Purification of Dihydrorobinetin from Robinia pseudoacacia L. Wood by Centrifugal Partition Chromatography. Separations 2016, 3, 23.

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