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Separations 2016, 3(4), 29; doi:10.3390/separations3040029

Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns

1
School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan
2
Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Alain Berthod
Received: 11 June 2016 / Revised: 21 August 2016 / Accepted: 26 August 2016 / Published: 22 September 2016
(This article belongs to the Special Issue Countercurrent Chromatography)
View Full-Text   |   Download PDF [2378 KB, uploaded 22 September 2016]   |  

Abstract

(1) Background: Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of peak resolution and shortening of separation time. (2) Methods: The long-pressed locular multilayer coils with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl sugar derivatives using a small-scale cross-axis coil planet centrifuge. (3) Results: Proteins were separated from each other and the separation was improved when the flow rate of the mobile phase was decreased from 0.8 to 0.4 mL/min. On the other hand, 4-methylumbelliferyl sugar derivatives were separated at the resolution of almost over 1.5 in short separation time under satisfactory stationary phase retention when the flow rate of the mobile phase was increased from 1.0 to 1.4 mL/min. (4) Conclusion: Better peak resolutions over the previous results were achieved using the long-pressed locular multilayer coil for proteins with aqueous two-phase systems (ATPS) and for 4-methylumbelliferyl sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule. View Full-Text
Keywords: countercurrent chromatography; cross-axis coil planet centrifuge; locular multilayer coil; partition efficiency; proteins; 4-methylumbelliferyl sugar derivatives countercurrent chromatography; cross-axis coil planet centrifuge; locular multilayer coil; partition efficiency; proteins; 4-methylumbelliferyl sugar derivatives
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Shinomiya, K.; Zaima, K.; Harikai, N.; Ito, Y. Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns. Separations 2016, 3, 29.

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