Table of Contents

Abstract: As the result of remarkable technological progress, this past decade has witnessed considerable advances in our ability to manipulate natural and engineered systems, particularly at the molecular level. These advancements offer the potential to revolutionize our world through the development of novel soft and hard materials and the construction of new cellular platforms for chemical and pharmaceutical synthesis. For these technologies to truly impact society, the development of process technology that will enable effective large-scale production is essential. Improved processes are also needed for more established technologies in chemical and biochemical manufacturing, as these industries face ever increasing competitive pressure that mandates continuous improvement. [...]

Abstract: We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (k_La) was 200%–400% higher in the HT tube than in a shake flask. Bacterial growth and plasmid production were 30% higher in the HT tube than in a 1-L Erlenmeyer flask. Mammalian cell cultures achieved a 25% higher cell density in the HT tube as compared to a 250-mL square-shaped bottle while insect cells grew as well in HT tubes as in 250-mL shake flasks. Because of their performance, disposability, and compact size, we conclude that 250-mL HT tubes are a useful alternative to other shaken containers for the cultivation of bacterial, mammalian and insect cells.

Abstract: In this paper we present the analogous electrical model for analyzing and determining the precise time dependence of concentrations in general first and zero order chemical reactions. In addition, the applicability of this analogous electrical model for investigating the optical and bio chemical processes is also presented. By constructing the proper analogous electrical circuit experimentally or with the help of special electrical software, the time behavior of the analyzed parameter even for extremely complicated processes can be obtained.