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Processes 2013, 1(1), 3-11; doi:10.3390/pr1010003

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, CH-1015 Lausanne, Switzerland
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Received: 15 December 2012 / Revised: 17 February 2013 / Accepted: 15 March 2013 / Published: 26 March 2013
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Abstract

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (kLa) was 200%–400% higher in the HT tube than in a shake flask. Bacterial growth and plasmid production were 30% higher in the HT tube than in a 1-L Erlenmeyer flask. Mammalian cell cultures achieved a 25% higher cell density in the HT tube as compared to a 250-mL square-shaped bottle while insect cells grew as well in HT tubes as in 250-mL shake flasks. Because of their performance, disposability, and compact size, we conclude that 250-mL HT tubes are a useful alternative to other shaken containers for the cultivation of bacterial, mammalian and insect cells.
Keywords: shake flasks; orbital shaking; bacteria; gas transfer; insect cells; mammalian cells shake flasks; orbital shaking; bacteria; gas transfer; insect cells; mammalian cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Tissot, S.; Michel, P.O.; Douet, C.J.; Grezet, S.; Baldi, L.; Hacker, D.L.; Wurm, F.M. Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures. Processes 2013, 1, 3-11.

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