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Processes 2013, 1(1), 3-11; doi:10.3390/pr1010003
Article

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

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Received: 15 December 2012; in revised form: 17 February 2013 / Accepted: 15 March 2013 / Published: 26 March 2013
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Abstract: We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (kLa) was 200%–400% higher in the HT tube than in a shake flask. Bacterial growth and plasmid production were 30% higher in the HT tube than in a 1-L Erlenmeyer flask. Mammalian cell cultures achieved a 25% higher cell density in the HT tube as compared to a 250-mL square-shaped bottle while insect cells grew as well in HT tubes as in 250-mL shake flasks. Because of their performance, disposability, and compact size, we conclude that 250-mL HT tubes are a useful alternative to other shaken containers for the cultivation of bacterial, mammalian and insect cells.
Keywords: shake flasks; orbital shaking; bacteria; gas transfer; insect cells; mammalian cells shake flasks; orbital shaking; bacteria; gas transfer; insect cells; mammalian cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tissot, S.; Michel, P.O.; Douet, C.J.; Grezet, S.; Baldi, L.; Hacker, D.L.; Wurm, F.M. Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures. Processes 2013, 1, 3-11.

AMA Style

Tissot S, Michel PO, Douet CJ, Grezet S, Baldi L, Hacker DL, Wurm FM. Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures. Processes. 2013; 1(1):3-11.

Chicago/Turabian Style

Tissot, Stéphanie; Michel, Patrik O.; Douet, Clara J.; Grezet, Sarah; Baldi, Lucia; Hacker, David L.; Wurm, Florian M. 2013. "Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures." Processes 1, no. 1: 3-11.


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