Next Article in Journal
Field-Amplified Sample Injection-Micellar Electrokinetic Chromatography for the Determination of Benzophenones in Food Simulants
Previous Article in Journal
Fractionation and Characterization of High Aspect Ratio Gold Nanorods Using Asymmetric-Flow Field Flow Fractionation and Single Particle Inductively Coupled Plasma Mass Spectrometry
Article Menu

Export Article

From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessArticle
Chromatography 2015, 2(3), 436-451; doi:10.3390/chromatography2030436

Poly(N-isopropylacrylamide) Hydrogels for Storage and Delivery of Reagents to Paper-Based Analytical Devices

Department of Chemistry and Biochemistry, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, CA 93407, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Carlos D. Garcia
Received: 4 May 2015 / Revised: 29 June 2015 / Accepted: 10 July 2015 / Published: 17 July 2015
(This article belongs to the Special Issue Microfluidic Paper-Based Analytical Devices)
View Full-Text   |   Download PDF [2519 KB, uploaded 17 July 2015]   |  

Abstract

The thermally responsive hydrogel N,N'-methylenebisacrylamide-cross-linked poly(N-isopropylacrylamide) (PNIPAM) was developed and evaluated as a reagent storage and delivery system for microfluidic paper-based analytical devices (microPADs). PNIPAM was shown to successfully deliver multiple solutions to microPADs in specific sequences or simultaneously in laminar-flow configuration and was found to be suitable for delivering four classes of reagents to the devices: Small molecules, enzymes, antibodies and DNA. PNIPAM was also able to successfully deliver a series of standard glucose solutions to microPADs equipped to perform a colorimetric glucose assay. The results of these tests were used to produce an external calibration curve, which in turn was used to determine the concentration of glucose in sample solutions. Finally, PNIPAM was used to store the enzyme horseradish peroxidase for 35 days under ambient conditions with no significant loss of activity. The combination of PNIPAM and microPADs may allow for more complex assays to be performed on paper-based devices, facilitate the preparation of external calibration curves in the field, and extend the shelf life of microPADs by stabilizing reagents in an easy-to-use format. View Full-Text
Keywords: paper; microfluidics; PNIPAM; microPAD paper; microfluidics; PNIPAM; microPAD
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mitchell, H.T.; Schultz, S.A.; Costanzo, P.J.; Martinez, A.W. Poly(N-isopropylacrylamide) Hydrogels for Storage and Delivery of Reagents to Paper-Based Analytical Devices. Chromatography 2015, 2, 436-451.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Chromatography EISSN 2227-9075 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top