Chromatography 2014, 1(2), 75-81; doi:10.3390/chromatography1020075
Article

An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green

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Received: 14 April 2014; in revised form: 15 May 2014 / Accepted: 16 May 2014 / Published: 27 May 2014
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Abstract: This paper describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leuco-malachite green (LMG) using an isocratic toxic organic solvent/reagent-free mobile phase. Chromatographic separations were performed an Inertsil® WP300 C4 with 0.02 mol/L octane sulfonic acid–ethanol mobile phase and a photodiode-array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. A harmless method for simultaneously detecting MG and LMG was developed and may be further applied to the quantification in foods.
Keywords: internal harmonized analytical method; malachite green; leuco-malachite green; high-performance liquid chromatography; photo-diode array
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Furusawa, N. An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green. Chromatography 2014, 1, 75-81.

AMA Style

Furusawa N. An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green. Chromatography. 2014; 1(2):75-81.

Chicago/Turabian Style

Furusawa, Naoto. 2014. "An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green." Chromatography 1, no. 2: 75-81.

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