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Biomedicines 2017, 5(2), 19; doi:10.3390/biomedicines5020019

Functionalisation of Polyvinylpyrrolidone on Gold Nanoparticles Enhances Its Anti-Amyloidogenic Propensity towards Hen Egg White Lysozyme

Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha 769008, India
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Author to whom correspondence should be addressed.
Academic Editor: Ashok K. Singh
Received: 2 March 2017 / Revised: 13 April 2017 / Accepted: 25 April 2017 / Published: 3 May 2017
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Abstract

Protein amyloids are characterized by aggregates that usually consist of fibres containing misfolded proteins and having a cross β-sheet conformation. These aggregates can eventually lead to several degenerative diseases like Alzheimer’s disease, amyotrophic lateral sclerosis (ALS), Huntington’s disease and Parkinson’s disease. The present study describes the effect of chemically synthesized polyvinylpyrrolidone (PVP)-conjugated gold nanoparticles (PVP-AuNps) on hen egg white lysozyme (HEWL) amyloids. The synthesized nanoparticles have been characterized using various biophysical techniques like Ultraviolet-Visible (UV-Vis) Spectroscopy, Transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, dynamic light scattering (DLS), zeta-potential measurement and Fourier transform infrared spectroscopy (FTIR). The aggregation studies showed that PVP acts as a partial inhibitor of HEWL amyloidogenesis. However, when conjugated to gold nanoparticle surface, it leads to complete inhibition of amyloid formation. Apart from inhibition, PVP-conjugated gold nanoparticles also exhibited a significant disaggregation effect on mature amyloids and hence can be exploited as an effective therapeutic agent against hereditary systemic amyloidosis. View Full-Text
Keywords: amyloid; thioflavint; gold nanoparticle; circular dichroism; lysozyme; disaggregation amyloid; thioflavint; gold nanoparticle; circular dichroism; lysozyme; disaggregation
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Das, T.; Kolli, V.; Karmakar, S.; Sarkar, N. Functionalisation of Polyvinylpyrrolidone on Gold Nanoparticles Enhances Its Anti-Amyloidogenic Propensity towards Hen Egg White Lysozyme. Biomedicines 2017, 5, 19.

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