Chemosensors 2017, 5(2), 16; doi:10.3390/chemosensors5020016
Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni
1
Surface Engineering and Nanotechnology Institute, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK
2
Biotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Kuala Lumpur P.O. Box 12301, Malaysia
3
Technical University of Berlin, Straße des 17. Juni 124, Berlin 10623, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Igor Medintz
Received: 31 March 2017 / Revised: 25 April 2017 / Accepted: 4 May 2017 / Published: 16 May 2017
(This article belongs to the Special Issue Biosensors Applications in Food Safety)
Abstract
Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 106 CFU·mL−1. The sensitivity of the assay was further enhanced to 4 × 104 CFU·mL−1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (106–107 CFU·mL−1). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni. View Full-TextKeywords:
Campylobacter jejuni; campylobacteriosis; Surface Plasmon Resonance (SPR); immunosensor; sandwich assay
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Masdor, N.A.; Altintas, Z.; Tothill, I.E. Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni. Chemosensors 2017, 5, 16.
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