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Chemosensors 2016, 4(3), 17; doi:10.3390/chemosensors4030017

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

1
Department of Analytical Chemistry, Institute of Chemistry, UNESP-Univ Estadual Paulista, Rua Francisco Degni, 55, Quitandinha, 14800-060 Araraquara, SP, Brazil
2
Department of Food and Nutrition, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Rodovia Araraquara Jaú, Km 01, s/n, Campos Ville, 14800-903 Araraquara, SP, Brazil
3
Department of Clinical Analysis, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Rodovia Araraquara Jaú, Km 01, s/n, Campos Ville, 14800-903 Araraquara, SP, Brazil
*
Authors to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Ligia Moretto
Received: 29 April 2016 / Revised: 22 June 2016 / Accepted: 19 August 2016 / Published: 1 September 2016
(This article belongs to the Special Issue Electrochemical Immunosensors and Aptasensors)
View Full-Text   |   Download PDF [1390 KB, uploaded 1 September 2016]   |  

Abstract

This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B1 (AFB1). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB1. The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB1 antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody–antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 × 10−9 to 1 × 10−7 mol·L−1 (1.56–31.2 ng·mL−1), limit of detection and limit of quantification, respectively, 3.6 × 10−10 and 1.1 × 10−9mol·L−1 (0.11 and 0.34 ng·mL−1). The proposed immunosensor was applied to analyze peanut samples. View Full-Text
Keywords: aflatoxin B1; immunosensor; electrochemical impedance spectroscopy aflatoxin B1; immunosensor; electrochemical impedance spectroscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Foguel, M.V.; Furlan Giordano, G.; de Sylos, C.M.; Carlos, I.Z.; Pupim Ferreira, A.A.; Benedetti, A.V.; Yamanaka, H. A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes. Chemosensors 2016, 4, 17.

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