Chemosensors 2016, 4(3), 11; doi:10.3390/chemosensors4030011
Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
Víctor Ruiz-Valdepeñas Montiel 1, Rebeca Magnolia Torrente-Rodríguez 1, Susana Campuzano 1,*
, Alessandro Pellicanò 2, Ángel Julio Reviejo 1, Maria Stella Cosio 2 and José Manuel Pingarrón 1,*
, Alessandro Pellicanò 2, Ángel Julio Reviejo 1, Maria Stella Cosio 2 and José Manuel Pingarrón 1,*
1
Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain
2
Department of Food, Environmental and Nutritional Sciences (DEFENS), University of Milan, Via Celoria 2, 20133 Milan, Italy
*
Authors to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Ligia Moretto
Received: 26 March 2016 / Revised: 5 June 2016 / Accepted: 24 June 2016 / Published: 28 June 2016
(This article belongs to the Special Issue Electrochemical Immunosensors and Aptasensors)
Abstract
In this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2) is reported. It involves the use of sandwich-type immunoassays using selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Amperometric detection at −0.20 V was performed using dual screen-printed carbon electrodes (SPdCEs) and the H2O2/hydroquinone (HQ) system. This methodology exhibits high sensitivity and selectivity for the target proteins providing detection limits of 18.0 and 0.07 ng/mL for Ara h 1 and Ara h 2, respectively, with an assay time of only 2 h. The usefulness of the approach was evaluated by detecting the endogenous content of both allergenic proteins in different food extracts as well as trace amounts of peanut allergen (0.0001% or 1.0 mg/kg) in wheat flour spiked samples. The developed platform provides better Low detection limits (LODs) in shorter assay times than those claimed for the allergen specific commercial ELISA kits using the same immunoreagents and quantitative information on individual food allergen levels. Moreover, the flexibility of the methodology makes it readily translate to the detection of other food-allergens. View Full-TextKeywords:
Ara h 1; Ara h 2; dual determination; magnetic beads; SPdCEs; amperometric immunosensor; food extracts
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MDPI and ACS Style
Ruiz-Valdepeñas Montiel, V.; Torrente-Rodríguez, R.M.; Campuzano, S.; Pellicanò, A.; Reviejo, Á.J.; Cosio, M.S.; Pingarrón, J.M. Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms. Chemosensors 2016, 4, 11.
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