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Chemosensors 2015, 3(2), 169-177; doi:10.3390/chemosensors3020169

Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Michael Ongaro
Received: 21 January 2015 / Accepted: 15 May 2015 / Published: 20 May 2015
(This article belongs to the Special Issue Electrochemical Sensors for Environmental and Food Analysis)
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Abstract

An alcohol dehydrogenase-based biosensor was prepared and tested for its use to determine ethanol in beer. The biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide and immobilized with a biocatalytic layer containing the enzyme. Function of the enzyme biosensor was tested in model ethanol samples, in which it showed a linear range of 15–120 g∙L−1 with a detection limit of 3.3 g∙L−1 (established as 3σ) and response time of 19 s. In a potential window from –0.2 to +0.45 V, interferences of both ascorbic and uric acids were negligible. Several types of marketed beers of Czech provenance were selected and subjected to measurements under optimized conditions but without any pretreatment of real samples. When compared with the reference method (gas chromatography), the results were in quite good agreement for beers of the pale lager type but higher contents of ethanol were indicated in the samples of dark lager beers. View Full-Text
Keywords: ethanol; amperometry; screen-printed carbon electrode; alcohol dehydrogenase; biosensor; beer ethanol; amperometry; screen-printed carbon electrode; alcohol dehydrogenase; biosensor; beer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Polan, V.; Eisner, A.; Vytřas, K. Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor. Chemosensors 2015, 3, 169-177.

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