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Proteomes 2018, 6(2), 18; https://doi.org/10.3390/proteomes6020018

Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans

1
Professur für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany
2
Lehrstuhl für Biochemie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany
3
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
*
Author to whom correspondence should be addressed.
Received: 16 March 2018 / Revised: 13 April 2018 / Accepted: 16 April 2018 / Published: 17 April 2018
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Abstract

Corynebacterium ulcerans is an emerging pathogen, which is increasingly recognized as an etiological agent of diphtheria, but can also evoke ulcers of the skin and systemic infections in humans. Besides man, the bacteria can colonize a wide variety of different animals, including cattle and pet animals, which might serve as a reservoir for human infections. In this study, surface-located proteins and the exoproteome of two Corynebacterium ulcerans strains were analyzed, since these may have key roles in the interaction of the pathogen with host cells. Strain 809 was isolated from a fatal case of human respiratory tract infection, while strain BR-AD22 was isolated from a nasal swap of an asymptomatic dog. While a very similar pattern of virulence factors was observed in the culture supernatant and surface protein fractions of the two strains, proteome analyses revealed a higher stability of 809 cells compared to strain BR-AD22. During exponential growth, 17% of encoded proteins of strain 809 were detectable in the medium, while 38% of the predicted proteins encoded by the BR-AD22 chromosome were found. Furthermore, the data indicate differential expression of phospholipase D and a cell wall-associated hydrolase, since these were only detected in strain BR-AD22. View Full-Text
Keywords: diphtheria; exoproteome; genomics; host-pathogen interaction; surface proteome; virulence factors diphtheria; exoproteome; genomics; host-pathogen interaction; surface proteome; virulence factors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bittel, M.; Gastiger, S.; Amin, B.; Hofmann, J.; Burkovski, A. Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans. Proteomes 2018, 6, 18.

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