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Proteomes 2018, 6(1), 13; https://doi.org/10.3390/proteomes6010013

Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean

1
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan
2
Faculty of Medicine, University of Tsukuba, Tsukuba 305-8577, Japan
*
Author to whom correspondence should be addressed.
Received: 10 January 2018 / Revised: 13 February 2018 / Accepted: 23 February 2018 / Published: 27 February 2018
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Abstract

Soybean, which is rich in protein and oil, is cultivated in several climatic zones; however, its growth is markedly decreased by flooding. Proteomics is a useful tool for understanding the flooding-response mechanism in soybean. Subcellular proteomics has the potential to elucidate localized cellular responses and investigate communications among subcellular components during plant growth and during stress. Under flooding, proteins related to signaling, stress and the antioxidative system are increased in the plasma membrane; scavenging enzymes for reactive-oxygen species are suppressed in the cell wall; protein translation is suppressed through inhibition of proteins related to preribosome biogenesis and mRNA processing in the nucleus; levels of proteins involved in the electron transport chain are reduced in the mitochondrion; and levels of proteins related to protein folding are decreased in the endoplasmic reticulum. This review discusses the advantages of a gel-free/label-free proteomic technique and methods of plant subcellular purification. It also summarizes cellular events in soybean under flooding and discusses future prospects for generation of flooding-tolerant soybean. View Full-Text
Keywords: subcellular proteomics; flooding-response mechanism; soybean subcellular proteomics; flooding-response mechanism; soybean
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Komatsu, S.; Hashiguchi, A. Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean. Proteomes 2018, 6, 13.

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