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Plants 2018, 7(3), 69; https://doi.org/10.3390/plants7030069

Inhibition of Lipid Peroxidation of Kiwicha (Amaranthus caudatus) Hydrolyzed Protein Using Zebrafish Larvae and Embryos

1
Laboratory of Functional Foods, Faculty of Foods Sciences and Engineering, Technical University of Ambato, Av. Los Chasquis y Rio Payamino, Campus Huachi, Ambato 1801334, Ecuador
2
CIT-RIO NEGRO Sede Atlántica, Universidad Nacional de Rio Negro (UNRN-CONICET), Don Bosco y Leloir s/n CP 8500, Rio Negro Viedma, Argentina
3
Department of Research, Faculty of Health Sciences, Technical University of Babahoyo, Av. Universitaria Km 21/2 Av. Montalvo., Babahoyo 120301, Ecuador
4
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79413, USA
5
Research Institute of Food Science (CIAL-CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 30 June 2018 / Revised: 25 August 2018 / Accepted: 29 August 2018 / Published: 2 September 2018
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Abstract

Amaranth protein concentrate (APC) was hydrolyzed under in vitro gastrointestinal conditions. APC proteins were partially degraded by pepsin at pHs 1.2, 2.0, and 3.2. During the intestinal phase (pepsin/pancreatin enzymes at pH 7.0), no polypeptide bands were observed in the gel, suggesting the susceptibility of amaranth proteins to the action of digestive enzymes. The potent in vitro inhibition of lipid peroxidation, shown by the gastric and intestinal digests, was confirmed in the zebrafish larvae, with a 72.86% reduction in oxidation of lipids in the presence of the gastric hydrolysate at pH 2.0, compared to a 95.72% reduction in the presence of the gastrointestinal digest. APC digests were capable of reducing reactive oxygen species (ROS) production in the zebrafish embryo model with a value of fluorescence of 52.5% for the gastric hydrolysate, and 48.4% for the intestinal hydrolysate. View Full-Text
Keywords: kiwicha; simulated gastrointestinal digestion; protein concentrate; lipid peroxidation; zebrafish embryos kiwicha; simulated gastrointestinal digestion; protein concentrate; lipid peroxidation; zebrafish embryos
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Vilcacundo, R.; Barrio, D.A.; Piñuel, L.; Boeri, P.; Tombari, A.; Pinto, A.; Welbaum, J.; Hernández-Ledesma, B.; Carrillo, W. Inhibition of Lipid Peroxidation of Kiwicha (Amaranthus caudatus) Hydrolyzed Protein Using Zebrafish Larvae and Embryos. Plants 2018, 7, 69.

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