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Plants 2015, 4(2), 298-319; doi:10.3390/plants4020298

Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway

Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India
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Academic Editors: Roberto Pilu and Francesca Sparvoli
Received: 28 February 2015 / Revised: 26 May 2015 / Accepted: 1 June 2015 / Published: 11 June 2015
(This article belongs to the Special Issue Phytic Acid Pathway and Breeding in Plants)
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Abstract

Phytic acid (PA) biosynthesis pathway genes were reported from multiple crop species. PA accumulation was enhanced during grain filling and at that time, hormones like Abscisic acid (ABA) and Gibberellic acid (GA3) interplay to control the process of seed development. Regulation of wheat PA pathway genes has not yet been reported in seeds. In an attempt to find the clues for the regulation by hormones, the promoter region of wheat PA pathway genes was analyzed for the presence of cis-elements. Multiple cis-elements of those known to be involved for ABA, GA3, salicylic acid (SA), and cAMP sensing were identified in the promoters of PA pathway genes. Eight genes (TaIMP, TaITPK1-4, TaPLC1, TaIPK2 and TaIPK1) involved in the wheat PA biosynthesis pathway were selected for the expression studies. The temporal expression response was studied in seeds treated with ABA and GA3 using quantitative real time PCR. Our results suggested that exogenous application of ABA induces few PA pathway genes in wheat grains. Comparison of expression profiles for PA pathway for GA3 and ABA suggested the antagonistic regulation of certain genes. Additionally, to reveal stress responses of wheat PA pathway genes, expression was also studied in the presence of SA and cAMP. Results suggested SA specific differential expression of few genes, whereas, overall repression of genes was observed in cAMP treated samples. This study is an effort to understand the regulation of PA biosynthesis genes in wheat. View Full-Text
Keywords: phytic acid; wheat; ABA; GA3; gene regulation; seed development phytic acid; wheat; ABA; GA3; gene regulation; seed development
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Aggarwal, S.; Shukla, V.; Bhati, K.K.; Kaur, M.; Sharma, S.; Singh, A.; Mantri, S.; Pandey, A.K. Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway. Plants 2015, 4, 298-319.

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