Certain Malvaceae Plants Have a Unique Accumulation of myo-Inositol 1,2,4,5,6-Pentakisphosphate
Abstract
:1. Introduction
2. Results and Discussion
2.1. Concentration of Inositol Phosphates in Leaf Extracts
2.2. Solid-Phase Extraction of Inositol Phosphate Concentrates
Plant | InsP5 Added | InsP6 Added | InsP5 Determined | InsP6 Determined | InsP5 Recovered | InsP6 Recovered |
---|---|---|---|---|---|---|
Tobacco | 0 µg | 0 µg | 0 µg | 37 µg | - | - |
Tobacco | 50 µg | 0 µg | 49 µg | 42 µg | 49 µg | - |
Cotton | 0 µg | 0 µg | 40 µg | 66 µg | - | - |
Cotton | 0 µg | 132 µg | 48 µg | 196 µg | - | 130 µg |
2.3. InsP6 and InsP5 Levels in Various Plants
Plant | Tissue | InsP6 (µM) | Ins(1,2,4,5,6)P5 (µM) |
---|---|---|---|
Zea mays | leaves | 101–198 (3) | nd |
Oryza sativa | leaves | 9–49 (6) | nd |
Arabidopsis thaliana | leaves | 29–89 (9) | 2–22 (5) |
Arabidopsis thaliana | seedlings | 47–110 (4) | nd |
Nicotiana tabacum | leaves | 11–24 (3) | nd |
Solanum lycopersicum | leaves | 39–65 (3) | nd |
Trifolium repens | leaves | 21 (1) | 6 (1) |
Acer rubrum | leaves | 35 (1) | 2 (1) |
Ginkgo biloba | leaves | 61 (1) | nd |
Camelina sativa | leaves | 70 (1) | 6 (1) |
Glycine max | leaves | nd (1) | nd |
Pueraria labata | leaves | nd (1) | nd |
Plant | Tissue | InsP6 (µM) | Ins(1,2,4,5,6)P5 (µM) |
---|---|---|---|
Gossypium hirsutum | leaves | 11–169 (6) | 5–510 (6) |
Gossypium hirsutum | seedlings | 77–140 (3) | 86–118 (3) |
Gossypium hirsutum | 14–17 dpa fiber | 43 (1) | nd |
Gossypium hirsutum | 16 dpa seeds | 105 (1) | nd |
Gossypium barbadense | leaves | 60–76 (2) | 329–507 (2) |
Gossypium raimondii | leaves | 71–86 (2) | 578–589 (2) |
Abelmoschus. esculentus | leaves | 90–336 (4) | 3 (1) |
Abelmoschus. esculentus | pod (husk) | 100 (1) | nd |
Abelmoschus manihot | leaves | 250–822 (4) | 2–7 (2) |
Alcea rosea | leaves | 50–52(2) | 10(2) |
Althea officinalis | leaves | 95–217 (2) | 38–74 (2) |
Hibiscus syriacus | leaves | 27–34 (3) | 10–19 (3) |
Hibiscus moscheutos | leaves | 39–73 (2) | 219–491 (2) |
Hibiscus rosa-sinensis | leaves | 31 (1) | 392 (1) |
Hibiscus cannabinus | leaves | 172–491 (5) | 19–57 (4) |
Hibiscus coccineus | leaves | 97–140 (2) | 536–647 (2) |
Hibiscus sabdariffa | leaves | 153 (1) | 114 (1) |
Hibiscus mutabilis | leaves | 49 (1) | 46 (1) |
InsP6 | InsP5 | InsP5:InsP6 | ||
---|---|---|---|---|
% of Total InsP | % of Total InsP | Ratio | ||
Arabidopsis | seedlings 1 | 27.8 | 2.70 | 0.097 |
G. hirsutum | seedling shoot | 1.18 | 4.35 | 3.7 |
G. hirsutum | seedling root | 3.78 | 7.20 | 1.9 |
G. hirsutum | young leaf | 9.47 | 7.07 | 0.75 |
Stage | Location | InsP6 (µM) | InsP5 (µM) |
---|---|---|---|
lowering | bottom near trunk | 11 | 5 |
flowering | middle near trunk | 20 | 14 |
flowering | middle near bolls | 33 | 126 |
flowering | top | 169 | 492 |
post-anthesis | top | 22 | 8 |
3. Experimental Section
3.1. Plant Material
3.2. Inositol Phosphate Standards
3.3. HCl Extraction and Concentration of Inositol Phosphates
3.4. Solid-Phase Extraction
3.5. HPIC
3.6. Recovery Experiments
3.7. Radiolabeling Plant Tissue with [3H]Inositol
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Phillippy, B.Q.; Perera, I.Y.; Donahue, J.L.; Gillaspy, G.E. Certain Malvaceae Plants Have a Unique Accumulation of myo-Inositol 1,2,4,5,6-Pentakisphosphate. Plants 2015, 4, 267-283. https://doi.org/10.3390/plants4020267
Phillippy BQ, Perera IY, Donahue JL, Gillaspy GE. Certain Malvaceae Plants Have a Unique Accumulation of myo-Inositol 1,2,4,5,6-Pentakisphosphate. Plants. 2015; 4(2):267-283. https://doi.org/10.3390/plants4020267
Chicago/Turabian StylePhillippy, Brian Q., Imara Y. Perera, Janet L. Donahue, and Glenda E. Gillaspy. 2015. "Certain Malvaceae Plants Have a Unique Accumulation of myo-Inositol 1,2,4,5,6-Pentakisphosphate" Plants 4, no. 2: 267-283. https://doi.org/10.3390/plants4020267