Next Article in Journal
Pistil Smut Infection Increases Ovary Production, Seed Yield Components, and Pseudosexual Reproductive Allocation in Buffalograss
Next Article in Special Issue
Molecular Composition of Plant Vacuoles: Important but Less Understood Regulations and Roles of Tonoplast Lipids
Previous Article in Journal
Redox Modulation Matters: Emerging Functions for Glutaredoxins in Plant Development and Stress Responses
Previous Article in Special Issue
Senescence-Associated Vacuoles, a Specific Lytic Compartment for Degradation of Chloroplast Proteins?
Article Menu

Export Article

Open AccessCommunication
Plants 2014, 3(4), 583-593; doi:10.3390/plants3040583

Plasmolysis: Loss of Turgor and Beyond

1
Cell Imaging and Ultrastructure Research, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
2
CR-Hana, Palacký University Olomouc, Šlechtitelů 586/11, 783 71 Olomouc-Holice, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 10 September 2014 / Revised: 15 October 2014 / Accepted: 14 November 2014 / Published: 26 November 2014
(This article belongs to the Special Issue Plant Vacuole)
View Full-Text   |   Download PDF [1419 KB, uploaded 26 November 2014]   |  

Abstract

Plasmolysis is a typical response of plant cells exposed to hyperosmotic stress. The loss of turgor causes the violent detachment of the living protoplast from the cell wall. The plasmolytic process is mainly driven by the vacuole. Plasmolysis is reversible (deplasmolysis) and characteristic to living plant cells. Obviously, dramatic structural changes are required to fulfill a plasmolytic cycle. In the present paper, the fate of cortical microtubules and actin microfilaments is documented throughout a plasmolytic cycle in living cells of green fluorescent protein (GFP) tagged Arabidopsis lines. While the microtubules became wavy and highly bundled during plasmolysis, cortical filamentous actin remained in close vicinity to the plasma membrane lining the sites of concave plasmolysis and adjusting readily to the diminished size of the protoplast. During deplasmolysis, cortical microtubule re-organization progressed slowly and required up to 24 h to complete the restoration of the original pre-plasmolytic pattern. Actin microfilaments, again, recovered faster and organelle movement remained intact throughout the whole process. In summary, the hydrostatic skeleton resulting from the osmotic state of the plant vacuole “overrules” the stabilization by cortical cytoskeletal elements. View Full-Text
Keywords: Arabidopsis hypocotyl; cytoskeleton; microtubules; actin microfilaments; plasmolysis; deplasmolysis; GFP-MAP4; GFP-TUA6; GFP-ABD Arabidopsis hypocotyl; cytoskeleton; microtubules; actin microfilaments; plasmolysis; deplasmolysis; GFP-MAP4; GFP-TUA6; GFP-ABD
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lang, I.; Sassmann, S.; Schmidt, B.; Komis, G. Plasmolysis: Loss of Turgor and Beyond. Plants 2014, 3, 583-593.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top