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Plants 2014, 3(3), 359-391; doi:10.3390/plants3030359

Expression of the Arabidopsis Sigma Factor SIG5 Is Photoreceptor and Photosynthesis Controlled

1
Plant Sciences, Heinrich-Heine-Universität, Universitätsstraße 1, Düsseldorf 40225, Germany
2
Plant Physiology, Freie Universität Berlin, Königin-Luise-Straße 12-16, Berlin 14195, Germany
*
Author to whom correspondence should be addressed.
Received: 31 May 2014 / Revised: 7 July 2014 / Accepted: 30 July 2014 / Published: 18 August 2014
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Abstract

Two collections of Arabidopsis GAL4 enhancer trap lines were screened for light-intensity dependent reporter gene activation. Line N9313 was isolated for its strong light-intensity regulation. The T-DNA element trapped distant enhancers of the SIG5 promoter, which drives expression of a sigma factor involved in regulation of chloroplast genes for photosystem II core proteins. The T-DNA insertion 715 bp upstream of the transcription initiation site splits the promoter in a distal and proximal part. Both parts are sensitive to blue and red light and depend on photosynthetic electron transport activity between photosystem II and the plastoquinone pool. The mainblue-light sensitivity is localized within a 196-bp sequence (–887 to –691 bp) in the proximal promoter region It is preferentially CRY1 and PHYB controlled. Type-I and type-II phytochromes mediate red-light sensitivity via various promoter elements spread over the proximal and distal upstream region. This work characterizes SIG5 as an anterograde control factor of chloroplast gene expression, which is controlled by chloroplast signals in a retrograde manner. View Full-Text
Keywords: SIG5; sigma factor; phytochrome; cryptochrome; photosynthesis; plastoquinone; photosystem II; retrograde signal SIG5; sigma factor; phytochrome; cryptochrome; photosynthesis; plastoquinone; photosystem II; retrograde signal
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Mellenthin, M.; Ellersiek, U.; Börger, A.; Baier, M. Expression of the Arabidopsis Sigma Factor SIG5 Is Photoreceptor and Photosynthesis Controlled. Plants 2014, 3, 359-391.

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