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Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds
AbstractIn our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC50 = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC50 = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells.
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Kai, H.; Akamatsu, E.; Torii, E.; Kodama, H.; Yukizaki, C.; Akagi, I.; Ino, H.; Sakakibara, Y.; Suiko, M.; Yamamoto, I.; Okayama, A.; Morishita, K.; Kataoka, H.; Matsuno, K. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants 2014, 3, 18-26.View more citation formats
Kai H, Akamatsu E, Torii E, Kodama H, Yukizaki C, Akagi I, Ino H, Sakakibara Y, Suiko M, Yamamoto I, Okayama A, Morishita K, Kataoka H, Matsuno K. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants. 2014; 3(1):18-26.Chicago/Turabian Style
Kai, Hisahiro; Akamatsu, Ena; Torii, Eri; Kodama, Hiroko; Yukizaki, Chizuko; Akagi, Isao; Ino, Hisatoshi; Sakakibara, Yoichi; Suiko, Masahito; Yamamoto, Ikuo; Okayama, Akihiko; Morishita, Kazuhiro; Kataoka, Hiroaki; Matsuno, Koji. 2014. "Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds." Plants 3, no. 1: 18-26.