Plants 2014, 3(1), 18-26; doi:10.3390/plants3010018
Article

Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds

1 Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka, Miyazaki 882-8508, Japan 2 Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan 3 Miyazaki Prefectural Food Research and Development Center, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan 4 Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan 5 Miyazaki Agricultural Experiment Station, 5805 Shimonaka, Sadowara-cho, Miyazaki, Miyazaki 880-0212, Japan 6 Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, Miyazaki 889-2192, Japan 7 Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan 8 Division of Tumor and Cellular Biochemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan 9 Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan
* Author to whom correspondence should be addressed.
Received: 31 October 2013; in revised form: 18 December 2013 / Accepted: 19 December 2013 / Published: 27 December 2013
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Abstract: In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC50 = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC50 = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells.
Keywords: adult T-cell leukemia; bitter gourd seed extract; α-eleostearic acid; phytohemagglutinin-activated human peripheral blood mononuclear cell

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MDPI and ACS Style

Kai, H.; Akamatsu, E.; Torii, E.; Kodama, H.; Yukizaki, C.; Akagi, I.; Ino, H.; Sakakibara, Y.; Suiko, M.; Yamamoto, I.; Okayama, A.; Morishita, K.; Kataoka, H.; Matsuno, K. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants 2014, 3, 18-26.

AMA Style

Kai H, Akamatsu E, Torii E, Kodama H, Yukizaki C, Akagi I, Ino H, Sakakibara Y, Suiko M, Yamamoto I, Okayama A, Morishita K, Kataoka H, Matsuno K. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants. 2014; 3(1):18-26.

Chicago/Turabian Style

Kai, Hisahiro; Akamatsu, Ena; Torii, Eri; Kodama, Hiroko; Yukizaki, Chizuko; Akagi, Isao; Ino, Hisatoshi; Sakakibara, Yoichi; Suiko, Masahito; Yamamoto, Ikuo; Okayama, Akihiko; Morishita, Kazuhiro; Kataoka, Hiroaki; Matsuno, Koji. 2014. "Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds." Plants 3, no. 1: 18-26.

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