Plants 2014, 3(1), 18-26; doi:10.3390/plants3010018
Article

Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds

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Received: 31 October 2013; in revised form: 18 December 2013 / Accepted: 19 December 2013 / Published: 27 December 2013
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Abstract: In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC50 = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC50 = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells.
Keywords: adult T-cell leukemia; bitter gourd seed extract; α-eleostearic acid; phytohemagglutinin-activated human peripheral blood mononuclear cell
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kai, H.; Akamatsu, E.; Torii, E.; Kodama, H.; Yukizaki, C.; Akagi, I.; Ino, H.; Sakakibara, Y.; Suiko, M.; Yamamoto, I.; Okayama, A.; Morishita, K.; Kataoka, H.; Matsuno, K. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants 2014, 3, 18-26.

AMA Style

Kai H, Akamatsu E, Torii E, Kodama H, Yukizaki C, Akagi I, Ino H, Sakakibara Y, Suiko M, Yamamoto I, Okayama A, Morishita K, Kataoka H, Matsuno K. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants. 2014; 3(1):18-26.

Chicago/Turabian Style

Kai, Hisahiro; Akamatsu, Ena; Torii, Eri; Kodama, Hiroko; Yukizaki, Chizuko; Akagi, Isao; Ino, Hisatoshi; Sakakibara, Yoichi; Suiko, Masahito; Yamamoto, Ikuo; Okayama, Akihiko; Morishita, Kazuhiro; Kataoka, Hiroaki; Matsuno, Koji. 2014. "Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds." Plants 3, no. 1: 18-26.

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