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New Compounds Induce Brassinosteroid Deficient-like Phenotypes in Rice
Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, 241-438, Shimoshinjo Nakano, Akita 010-0195, Japan
* Author to whom correspondence should be addressed.
Received: 29 May 2013; in revised form: 5 August 2013 / Accepted: 8 August 2013 / Published: 13 August 2013
Abstract: Brassinosteroids (BRs) are steroidal plant hormones with potent plant growth promoting activity. Because BR-deficient mutants of rice exhibit altered plant architecture and important agronomic traits, we conducted a systemic search for specific inhibitors of BR biosynthesis to manipulate the BR levels in plant tissues. Although previous studies have been conducted with BR biosynthesis inhibitors in dicots, little is known regarding the effects of BR biosynthesis inhibition in monocot plants. In this work, we used potent inhibitors of BR biosynthesis in Arabidopsis, and we performed a hydroponic culture of rice seedlings to evaluate the effects of BR biosynthesis inhibition. Among the test compounds, we found that 1-[[2-(4-Chlorophenyl)-4-(phenoxymethyl)-1,3-dioxolan-2-yl]methyl]-1H-1,2,4-triazole (1) is a potent inhibitor that could induce phenotypes in rice seedlings that were similar to those observed in brassinosteroid deficient plants. The IC50 value for the retardation of plant growth in rice seedlings was approximately 1.27 ± 0.43 μM. The IC50 value for reducing the bending angle of the lamina joint was approximately 0.55 ± 0.15 μM.
Keywords: brassinosteroid biosynthesis inhibitor; plant hormone; azole derivatives
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MDPI and ACS Style
Matsumoto, T.; Yamada, K.; Iwasaki, I.; Yoshizawa, Y.; Oh, K. New Compounds Induce Brassinosteroid Deficient-like Phenotypes in Rice. Plants 2013, 2, 521-529.
Matsumoto T, Yamada K, Iwasaki I, Yoshizawa Y, Oh K. New Compounds Induce Brassinosteroid Deficient-like Phenotypes in Rice. Plants. 2013; 2(3):521-529.
Matsumoto, Tadashi; Yamada, Kazuhiro; Iwasaki, Ikuko; Yoshizawa, Yuko; Oh, Keimei. 2013. "New Compounds Induce Brassinosteroid Deficient-like Phenotypes in Rice." Plants 2, no. 3: 521-529.