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Plants 2013, 2(3), 530-540; doi:10.3390/plants2030530
Article

Identification of CP12 as a Novel Calcium-Binding Protein in Chloroplasts

1,†,*  and 1,2
Received: 11 July 2013; in revised form: 8 August 2013 / Accepted: 19 August 2013 / Published: 26 August 2013
(This article belongs to the Special Issue Calcium Signaling in Plants)
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Abstract: Calcium plays an important role in the regulation of several chloroplast processes. However, very little is still understood about the calcium fluxes or calcium-binding proteins present in plastids. Indeed, classical EF-hand containing calcium-binding proteins appears to be mostly absent from plastids. In the present study we analyzed the stroma fraction of Arabidopsis chloroplasts for the presence of novel calcium-binding proteins using 2D-PAGE separation followed by calcium overlay assay. A small acidic protein was identified by mass spectrometry analyses as the chloroplast protein CP12 and the ability of CP12 to bind calcium was confirmed with recombinant proteins. CP12 plays an important role in the regulation of the Calvin-Benson-Bassham Cycle participating in the assembly of a supramolecular complex between phosphoribulokinase and glyceraldehyde 3-phosphate dehydrogenase, indicating that calcium signaling could play a role in regulating carbon fixation.
Keywords: chloroplast; calcium signaling; CP12; Calvin-Benson-Bassham Cycle chloroplast; calcium signaling; CP12; Calvin-Benson-Bassham Cycle
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Rocha, A.G.; Vothknecht, U.C. Identification of CP12 as a Novel Calcium-Binding Protein in Chloroplasts. Plants 2013, 2, 530-540.

AMA Style

Rocha AG, Vothknecht UC. Identification of CP12 as a Novel Calcium-Binding Protein in Chloroplasts. Plants. 2013; 2(3):530-540.

Chicago/Turabian Style

Rocha, Agostinho G.; Vothknecht, Ute C. 2013. "Identification of CP12 as a Novel Calcium-Binding Protein in Chloroplasts." Plants 2, no. 3: 530-540.

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