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Plants 2013, 2(3), 429-440; doi:10.3390/plants2030429
Article

Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube

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Received: 28 March 2013; in revised form: 3 June 2013 / Accepted: 18 June 2013 / Published: 25 June 2013
(This article belongs to the Special Issue Pollen Tube Growth)
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Abstract: The influence of three different pollen germination media on the transcript profile of Arabidopsis pollen tubes has been assessed by real-time PCR on a selection of cell wall related genes, and by a statistical analysis of microarray Arabidopsis pollen tube data sets. The qPCR assays have shown remarkable differences on the transcript levels of specific genes depending upon the formulation of the germination medium used. With the aid of principal component analysis performed on existing microarray data, a subset of genes has been identified that is more prone to produce diverging transcript levels. A functional classification of those genes showed that the clusters with higher number of members were those for hydrolase activity (based in molecular function) and for cell wall (based in cellular component). Taken together, these results may indicate that the nutrient composition of the pollen germination media influences pollen tube metabolism and that caution must be taken when interpreting transcriptomic data of pollen tubes.
Keywords: Arabidopsis thaliana; pollen tube; pollen tube growth media; Arabinogalactan proteins; real-time PCR Arabidopsis thaliana; pollen tube; pollen tube growth media; Arabinogalactan proteins; real-time PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

da Costa, M.L.; Pereira, L.G.; Coimbra, S. Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube. Plants 2013, 2, 429-440.

AMA Style

da Costa ML, Pereira LG, Coimbra S. Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube. Plants. 2013; 2(3):429-440.

Chicago/Turabian Style

da Costa, Mário L.; Pereira, Luís G.; Coimbra, Sílvia. 2013. "Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube." Plants 2, no. 3: 429-440.

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