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Biomolecules 2016, 6(1), 8; doi:10.3390/biom6010008

Functional Analysis of the Glucuronyltransferases GlcAT-P and GlcAT-S of Drosophila melanogaster: Distinct Activities towards the O-linked T-antigen

1
Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann Str. 52, Cologne 50931, Germany
2
Octapharma Biopharmaceuticals GmbH, Im Neuenheimer Feld 590, Heidelberg 69220, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Hans Vliegenthart
Received: 26 May 2015 / Revised: 21 October 2015 / Accepted: 22 November 2015 / Published: 6 January 2016
(This article belongs to the Special Issue Challenges in Glycan, Glycoprotein and Proteoglycan Research)
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Abstract

The Drosophila melanogaster glucuronyltransferases dGlcAT-S and dGlcAT-P were reported to be expressed ubiquitously and results of in vitro activity assays indicate a functional redundancy. We analyzed both transferases in vivo and in vitro and could show significant differences in their activity towards N-and O-glycoproteins in vivo. While GlcAT-P is able to use N-linked N-acetyllactosamine chains and the O-linked T-antigen as a substrate to form non-sulfated HNK1- (GlcAβ1-3Galβ1-4GlcNAcβ1-) and glucuronyl-T-antigens in vivo, GlcAT-S adds glucuronic acid only to N-linked chains, thereby synthesizing only the non-sulfated HNK1-antigen. View Full-Text
Keywords: glucuronyltransferases; Drosophila melanogaster; N-glycans; O-glycans; mass spectrometry; glycomics glucuronyltransferases; Drosophila melanogaster; N-glycans; O-glycans; mass spectrometry; glycomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Breloy, I.; Schwientek, T.; Althoff, D.; Holz, M.; Koppen, T.; Krupa, A.; Hanisch, F.-G. Functional Analysis of the Glucuronyltransferases GlcAT-P and GlcAT-S of Drosophila melanogaster: Distinct Activities towards the O-linked T-antigen. Biomolecules 2016, 6, 8.

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