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Biomolecules 2014, 4(2), 402-418; doi:10.3390/biom4020402
Review

Design of Catalytically Amplified Sensors for Small Molecules

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Received: 6 February 2014; in revised form: 21 March 2014 / Accepted: 26 March 2014 / Published: 17 April 2014
(This article belongs to the Special Issue Metal Binding Proteins)
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Abstract: Catalytically amplified sensors link an allosteric analyte binding site with a reactive site to catalytically convert substrate into colored or fluorescent product that can be easily measured. Such an arrangement greatly improves a sensor’s detection limit as illustrated by successful application of ELISA-based approaches. The ability to engineer synthetic catalytic sites into non-enzymatic proteins expands the repertoire of analytes as well as readout reactions. Here we review recent examples of small molecule sensors based on allosterically controlled enzymes and organometallic catalysts. The focus of this paper is on biocompatible, switchable enzymes regulated by small molecules to track analytes both in vivo and in the environment.
Keywords: sensor; protein design; signal amplification; catalysis sensor; protein design; signal amplification; catalysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Makhlynets, O.V.; Korendovych, I.V. Design of Catalytically Amplified Sensors for Small Molecules. Biomolecules 2014, 4, 402-418.

AMA Style

Makhlynets OV, Korendovych IV. Design of Catalytically Amplified Sensors for Small Molecules. Biomolecules. 2014; 4(2):402-418.

Chicago/Turabian Style

Makhlynets, Olga V.; Korendovych, Ivan V. 2014. "Design of Catalytically Amplified Sensors for Small Molecules." Biomolecules 4, no. 2: 402-418.


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