Biomolecules 2013, 3(4), 733-740; doi:10.3390/biom3040733
Article

Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni

Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA
* Author to whom correspondence should be addressed.
Received: 29 August 2013; in revised form: 18 September 2013 / Accepted: 18 September 2013 / Published: 30 September 2013
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Abstract: From the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-(3-O-β-D-glucopyranosyl-α-L-rhamnopyranosyl)-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester] (1); also known as rebaudioside O having seven sugar units has been isolated. Its structural characterization has been achieved by the extensive 1D (1H and 13C), and 2D NMR (COSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside O using acid and enzymatic methods to identify aglycone and sugar residues in its structure as well as their configurations.
Keywords: Stevia rebaudiana; diterpene glycoside; isolation; structure elucidation; spectral data; hydrolysis studies

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MDPI and ACS Style

Chaturvedula, V.S.P.; Chen, S.; Yu, O.; Mao, G. Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni . Biomolecules 2013, 3, 733-740.

AMA Style

Chaturvedula VSP, Chen S, Yu O, Mao G. Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni . Biomolecules. 2013; 3(4):733-740.

Chicago/Turabian Style

Chaturvedula, Venkata S.P.; Chen, Steven; Yu, Oliver; Mao, Guohong. 2013. "Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni ." Biomolecules 3, no. 4: 733-740.

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