Biomolecules 2013, 3(1), 18-38; doi:10.3390/biom3010018
Article

Comparative Ultrastructure and Carbohydrate Composition of Gastroliths from Astacidae, Cambaridae and Parastacidae Freshwater Crayfish (Crustacea, Decapoda)

1,†,* email, 2email, 3email, 1email, 1email, 4email, 4email and 2email
Received: 25 October 2012; in revised form: 27 November 2012 / Accepted: 28 November 2012 / Published: 21 December 2012
(This article belongs to the Special Issue Challenges in Glycan, Glycoprotein and Proteoglycan Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Crustaceans have to cyclically replace their rigid exoskeleton in order to grow. Most of them harden this skeleton by a calcification process. Some decapods (land crabs, lobsters and crayfish) elaborate calcium storage structures as a reservoir of calcium ions in their stomach wall, as so-called gastroliths. For a better understanding of the cyclic elaboration of these calcium deposits, we studied the ultrastructure of gastroliths from freshwater crayfish by using a combination of microscopic and physical techniques. Because sugars are also molecules putatively involved in the elaboration process of these biomineralizations, we also determined their carbohydrate composition. This study was performed in a comparative perspective on crayfish species belonging to the infra-order Astacidea (Decapoda, Malacostraca): three species from the Astacoidea superfamily and one species from the Parastacoidea superfamily. We observed that all the gastroliths exhibit a similar dense network of protein-chitin fibers, from macro- to nanoscale, within which calcium is precipitated as amorphous calcium carbonate. Nevertheless, they are not very similar at the molecular level, notably as regards their carbohydrate composition. Besides glucosamine, the basic carbohydrate component of chitin, we evidenced the presence of other sugars, some of which are species-specific like rhamnose and galacturonic acid whereas xylose and mannose could be linked to proteoglycan components.
Keywords: biomineralization; calcification; calcium storage; carbohydrates; crayfish; Crustacea; gastrolith; organic matrix; proteoglycans
PDF Full-text Download PDF Full-Text [1427 KB, uploaded 21 December 2012 15:35 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Luquet, G.; Fernández, M.S.; Badou, A.; Guichard, N.; Roy, N.L.; Corneillat, M.; Alcaraz, G.; Arias, J.L. Comparative Ultrastructure and Carbohydrate Composition of Gastroliths from Astacidae, Cambaridae and Parastacidae Freshwater Crayfish (Crustacea, Decapoda). Biomolecules 2013, 3, 18-38.

AMA Style

Luquet G, Fernández MS, Badou A, Guichard N, Roy NL, Corneillat M, Alcaraz G, Arias JL. Comparative Ultrastructure and Carbohydrate Composition of Gastroliths from Astacidae, Cambaridae and Parastacidae Freshwater Crayfish (Crustacea, Decapoda). Biomolecules. 2013; 3(1):18-38.

Chicago/Turabian Style

Luquet, Gilles; Fernández, María S.; Badou, Aïcha; Guichard, Nathalie; Roy, Nathalie L.; Corneillat, Marion; Alcaraz, Gérard; Arias, José L. 2013. "Comparative Ultrastructure and Carbohydrate Composition of Gastroliths from Astacidae, Cambaridae and Parastacidae Freshwater Crayfish (Crustacea, Decapoda)." Biomolecules 3, no. 1: 18-38.

Biomolecules EISSN 2218-273X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert