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Biomolecules 2012, 2(4), 549-563; doi:10.3390/biom2040549

Hyaluronidases Have Strong Hydrolytic Activity toward Chondroitin 4-Sulfate Comparable to that for Hyaluronan

1
Laboratory of Proteoglycan Signaling and Therapeutics, Hokkaido University Graduate School of Life Science, Sapporo 001-0021, Japan
2
Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Nagoya 468-8503, Japan
*
Author to whom correspondence should be addressed.
Received: 13 October 2012 / Revised: 22 October 2012 / Accepted: 8 November 2012 / Published: 12 November 2012
(This article belongs to the Special Issue Challenges in Glycan, Glycoprotein and Proteoglycan Research)
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Abstract

Chondroitin sulfate (CS) chains are involved in the regulation of various biological processes. However, the mechanism underlying the catabolism of CS is not well understood. Hyaluronan (HA)-degrading enzymes, the hyaluronidases, are assumed to act at the initial stage of the degradation process, because HA is similar in structure to nonsulfated CS, chondroitin (Chn). Although human hyaluronidase-1 (HYAL1) and testicular hyaluronidase (SPAM1) can degrade not only HA but also CS, they are assumed to digest CS to only a limited extent. In this study, the hydrolytic activities of HYAL1 and SPAM1 toward CS-A, CS-C, Chn, and HA were compared. HYAL1 depolymerized CS-A and HA to a similar extent. SPAM1 degraded CS-A, Chn, and HA to a similar extent. CS is widely distributed from very primitive organisms to humans, whereas HA has been reported to be present only in vertebrates with the single exception of a mollusk. Therefore, a genuine substrate of hyaluronidases appears to be CS as well as HA.
Keywords: 2AB: 2-aminobenzamide; Chn: chondroitin; CS: chondroitin sulfate; HA: hyaluronan; HPLC: high performance liquid chromatography 2AB: 2-aminobenzamide; Chn: chondroitin; CS: chondroitin sulfate; HA: hyaluronan; HPLC: high performance liquid chromatography
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Honda, T.; Kaneiwa, T.; Mizumoto, S.; Sugahara, K.; Yamada, S. Hyaluronidases Have Strong Hydrolytic Activity toward Chondroitin 4-Sulfate Comparable to that for Hyaluronan. Biomolecules 2012, 2, 549-563.

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