Biomolecules 2012, 2(4), 549-563; doi:10.3390/biom2040549
Article

Hyaluronidases Have Strong Hydrolytic Activity toward Chondroitin 4-Sulfate Comparable to that for Hyaluronan

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Received: 13 October 2012; in revised form: 22 October 2012 / Accepted: 8 November 2012 / Published: 12 November 2012
(This article belongs to the Special Issue Challenges in Glycan, Glycoprotein and Proteoglycan Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Chondroitin sulfate (CS) chains are involved in the regulation of various biological processes. However, the mechanism underlying the catabolism of CS is not well understood. Hyaluronan (HA)-degrading enzymes, the hyaluronidases, are assumed to act at the initial stage of the degradation process, because HA is similar in structure to nonsulfated CS, chondroitin (Chn). Although human hyaluronidase-1 (HYAL1) and testicular hyaluronidase (SPAM1) can degrade not only HA but also CS, they are assumed to digest CS to only a limited extent. In this study, the hydrolytic activities of HYAL1 and SPAM1 toward CS-A, CS-C, Chn, and HA were compared. HYAL1 depolymerized CS-A and HA to a similar extent. SPAM1 degraded CS-A, Chn, and HA to a similar extent. CS is widely distributed from very primitive organisms to humans, whereas HA has been reported to be present only in vertebrates with the single exception of a mollusk. Therefore, a genuine substrate of hyaluronidases appears to be CS as well as HA.
Keywords: 2AB: 2-aminobenzamide; Chn: chondroitin; CS: chondroitin sulfate; HA: hyaluronan; HPLC: high performance liquid chromatography
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MDPI and ACS Style

Honda, T.; Kaneiwa, T.; Mizumoto, S.; Sugahara, K.; Yamada, S. Hyaluronidases Have Strong Hydrolytic Activity toward Chondroitin 4-Sulfate Comparable to that for Hyaluronan. Biomolecules 2012, 2, 549-563.

AMA Style

Honda T, Kaneiwa T, Mizumoto S, Sugahara K, Yamada S. Hyaluronidases Have Strong Hydrolytic Activity toward Chondroitin 4-Sulfate Comparable to that for Hyaluronan. Biomolecules. 2012; 2(4):549-563.

Chicago/Turabian Style

Honda, Tomoko; Kaneiwa, Tomoyuki; Mizumoto, Shuji; Sugahara, Kazuyuki; Yamada, Shuhei. 2012. "Hyaluronidases Have Strong Hydrolytic Activity toward Chondroitin 4-Sulfate Comparable to that for Hyaluronan." Biomolecules 2, no. 4: 549-563.

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