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Biomolecules 2012, 2(1), 122-142; doi:10.3390/biom2010122
Article

Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression

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Received: 30 December 2011; in revised form: 4 February 2012 / Accepted: 14 February 2012 / Published: 23 February 2012
(This article belongs to the Special Issue Feature Papers)
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Abstract: Protein tyrosine phosphatase interacting protein 51 (PTPIP51), also known as regulator of microtubule dynamics protein 3, was identified as an in vitro and in vivo interaction partner of CGI-99 and Nuf-2. PTPIP51 mRNA is expressed in all stages of the cell cycle; it is highly expressed six hours post-nocodazole treatment and minimally expressed one hour post-nocodazole treatment. Recent investigations located PTPIP51 protein at the equatorial plate. This study reports the localization of the PTPIP51/CGI-99 and the PTPIP51/Nuf-2 complex at the equatorial region during mitosis. Moreover, Duolink proximity ligation assays revealed an association of PTPIP51 with the microtubular cytoskeleton and the spindle apparatus. High amounts of phosphorylated PTPIP51 associated with the spindle poles was seen by confocal microscopy. In parallel a strong interaction of PTPIP51 with the epidermal growth factor receptor phosphorylating PTPIP51 at the tyrosine 176 residue was seen. In the M/G1 transition a high level of interaction between PTPIP51 and PTP1B was registered, thus restoring the interaction of PTPIP51 and Raf-1, depleted in mitotic cells. Summarizing these new facts, we conclude that PTPIP51 is necessary for normal mitotic processes, impacting on chromosomal division and control of the MAPK pathway activity.
Keywords: PTPIP51; FAM82A2; FAM82C; RMD-3; mitosis; cell cycle; microtubule; CGI-99; Nuf-2; EGFR PTPIP51; FAM82A2; FAM82C; RMD-3; mitosis; cell cycle; microtubule; CGI-99; Nuf-2; EGFR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Brobeil, A.; Graf, M.; Eiber, M.; Wimmer, M. Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression. Biomolecules 2012, 2, 122-142.

AMA Style

Brobeil A, Graf M, Eiber M, Wimmer M. Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression. Biomolecules. 2012; 2(1):122-142.

Chicago/Turabian Style

Brobeil, Alexander; Graf, Michaela; Eiber, Moritz; Wimmer, Monika. 2012. "Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression." Biomolecules 2, no. 1: 122-142.



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