Galectin-1 Cooperates with Yersinia Outer Protein (Yop) P to Thwart Protective Immunity by Repressing Nitric Oxide Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Culture and Purification of Yops
2.2. Mice and Infection
2.3. Stimulation of Peritoneal Macrophages
2.4. NO and Urea Determination
2.5. Apoptosis Assays
2.6. Preparation and Purification of RGal1
2.7. ELISA Assays
2.8. Oxidative Burst Assay
2.9. In Vivo and In Vitro Supplementation of RGal1
2.10. Analysis of Yops-Gal1 Interactions by SDS-PAGE and Western Blot
2.11. Lectin Blotting
2.12. Yops Proteolysis Using Trypsin Digestion
2.13. Schiff Staining
2.14. Flow Cytometry
2.15. Mass Spectrometry
2.16. Bioinformatics Analysis
2.17. Western Blot Analysis of INOS Expression
2.18. Statistical Analysis
3. Results
3.1. Galectin-1 Binds to YopP in Y. enterocolitica
3.2. Galectin-1 Recognizes Yops in a Carbohydrate-Dependent Manner
3.3. Mass Spectrometry-Based Proteomics Analysis of Yops
3.4. Gal1 Protects Yops from Protease Degradation
3.5. Gal1 and YopP Control Y. enterocolitica Infection by Decreasing NO Production
3.6. Exogenous Supplementation of rGal1 Does Not Influence the Protective Anti-Y. enterocolitica Response Observed in the Absence of YopP
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Lectin | Ligands Described | Reference |
---|---|---|
Arachis Hypogaea (Peanut agglutinin) (PNA) | Galβ(1–3)GalNAc Galβ(1–3)GlcNAc Galβ(1–4)GlcNAc Lactose Galactose | [55] |
Erythrina crystagalli (ECA) | Galβ(1–4)GlcNAc Lactose > GalNAc > Gal | [56] |
Observed a | Mass Expt b | Mass (Theor) c | Delta Error (Da) d | Pep_exp_z e | Start f | End g | Peptide Sequence h | Modifications i |
---|---|---|---|---|---|---|---|---|
707.816 | 2120.4261 | 2120.0208 | 0.4053 | +3 | 232 | 250 | LNEYLNTNPQGVGTVVNKK | Deamidated (NQ); Lys->CamCys (K) |
1123.8687 | 3368.5843 | 3367.5 | 1.0843 | +3 | 100 | 122 | FIINMGEGGIHFSVIDYKHINGK | Deamidated (NQ); Lys->CamCys (K); Hex(1)HexNAc(1) (N); Hex(1)HexNAc(1) (ST); Oxidation (M) |
1344.4664 | 2686.9182 | 2686.2353 | 0.6829 | +2 | 48 | 67 | NYSRLDIEVMPALVIQANNK | Deamidated (NQ); Hex(1)HexNAc(1) (N); Lys->CamCys (K) |
954.9102 | 2861.7088 | 2861.3441 | 0.3647 | +3 | 258 | 276 | FDNNKSIIDGKELSVSVHK | Deamidated (NQ); 2 Hex(1)HexNAc(1) (ST) |
470.2974 | 938.5802 | 937.5219 | 1.0583 | +2 | 284 | 288 | TLLKV | Hex(1)HexNAc(1) (ST) |
604.0027 | 1205.9908 | 1205.5122 | 0.4787 | +2 | 182 | 187 | KLYTER | Hex(1)HexNAc(1) (ST); Lys->CamCys (K) |
559.2594 | 1674.7563 | 1673.6991 | 1.0572 | +3 | 250 | 262 | KNETIFNRFDNNK | Deamidated (NQ); Lys->CamCys (K) |
599.2961 | 1794.8664 | 1793.7499 | 1.1165 | +3 | 21 | 29 | SLISNEELK | Hex(1)HexNAc(1) (N); Hex(1)HexNAc(1) (ST); Lys->CamCys (K) |
761.4185 | 1520.8224 | 1520.7471 | 0.0754 | +2 | 200 | 213 | GILSDSENPLPHNK | Deamidated (NQ) |
515.7982 | 1544.3728 | 1544.6201 | −0.2473 | +3 | 251 | 262 | NETIFNRFDNNK | Deamidated (NQ); Lys->CamCys (K) |
1092.9822 | 3275.9248 | 3275.4749 | 0.4499 | +3 | 1 | 20 | MIGPISQINSFGGLSEKETR | Deamidated (NQ); Hex(1)HexNAc(1) (N); 2 Hex(1)HexNAc(1) (ST); Oxidation (M) |
525.9216 | 1574.7429 | 1574.7069 | 0.036 | +3 | 263 | 276 | SIIDGKELSVSVHK | Lys->CamCys (K) |
525.2371 | 2621.1492 | 2620.2921 | 0.8571 | +5 | 30 | 51 | NIIIQLETDIADGSWFHKNYSR | Deamidated (NQ) |
890.7302 | 3558.8918 | 3558.7498 | 0.142 | +4 | 1 | 29 | MIGPISQINSFGGLSEKETRSLISNEELK | Deamidated (NQ); Hex(1)HexNAc(1) (N); Oxidation (M) |
532.2823 | 1062.55 | 1062.5193 | 0.0307 | +2 | 225 | 230 | HTQGKK | Hex(1)HexNAc(1) (ST) |
533.9495 | 1598.8266 | 1598.8767 | −0.05 | +1 | 278 | 288 | RIAEYKTLLK | Hex(1)HexNAc(1) (ST) |
793.7171 | 2378.1295 | 2378.0999 | 0.0296 | +2 | 2 | 17 | IGPISQINSFGGLSEK | Deamidated (NQ); 2 Hex(1)HexNAc(1) (ST) |
642.8429 | 1283.6713 | 1282.4918 | 1.1795 | +3 | 225 | 230 | HTQGKKR | Hex(1)HexNAc(1) (ST); 2 Lys->CamCys (K) |
793.4021 | 2377.1845 | 2376.9625 | 0.2219 | +3 | 258 | 268 | FDNNKSIIDGK | Hex(1)HexNAc(1) (N); Hex(1)HexNAc(1) (ST); Lys->CamCys (K) |
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Jofre, B.L.; Eliçabe, R.J.; Silva, J.E.; Pérez Sáez, J.M.; Paez, M.D.; Callegari, E.; Mariño, K.V.; Di Genaro, M.S.; Rabinovich, G.A.; Davicino, R.C. Galectin-1 Cooperates with Yersinia Outer Protein (Yop) P to Thwart Protective Immunity by Repressing Nitric Oxide Production. Biomolecules 2021, 11, 1636. https://doi.org/10.3390/biom11111636
Jofre BL, Eliçabe RJ, Silva JE, Pérez Sáez JM, Paez MD, Callegari E, Mariño KV, Di Genaro MS, Rabinovich GA, Davicino RC. Galectin-1 Cooperates with Yersinia Outer Protein (Yop) P to Thwart Protective Immunity by Repressing Nitric Oxide Production. Biomolecules. 2021; 11(11):1636. https://doi.org/10.3390/biom11111636
Chicago/Turabian StyleJofre, Brenda Lucila, Ricardo Javier Eliçabe, Juan Eduardo Silva, Juan Manuel Pérez Sáez, Maria Daniela Paez, Eduardo Callegari, Karina Valeria Mariño, María Silvia Di Genaro, Gabriel Adrián Rabinovich, and Roberto Carlos Davicino. 2021. "Galectin-1 Cooperates with Yersinia Outer Protein (Yop) P to Thwart Protective Immunity by Repressing Nitric Oxide Production" Biomolecules 11, no. 11: 1636. https://doi.org/10.3390/biom11111636